FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308011
Sequences flagged as poor quality0
Sequence length125
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG154535.017028612614484No Hit
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT134144.355039268078088No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT124564.044011415176731No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA123934.023557600215576No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA120903.925184490164312No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT119203.8699916561421506No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA117833.8255127251948795No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA108293.51578352721169No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG107003.4739019061007563No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC101523.2959861823116707No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA98903.2109242851716333No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC98423.195340426153612No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC97173.1547574599608454No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC95243.092097360159215No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC94943.082357448272951No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT93733.043073136998354No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC79002.5648434633828012No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA71322.315501719094448No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT71132.309333108233148No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA70642.2934245854855835No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC65472.125573437312304No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA63342.056420062919831No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG41471.3463804864111997No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG36881.1973598345513634No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCTGCCCTCAACAAGATG17220.5590709422715423No Hit
CAATGGTTCACTGAAGACCCAAACTCCTGGCCCCTGTCATGTAGAACCAT14560.47271039021333655No Hit
TGACTGCTCTTTTCACCCATCTTACGGTAGCAGATGAAGCTCCCAGAATG12740.4136215914366695No Hit
CAATGGTTCACTGAAGACCCAGGTCAGGAGCCACTTGCCACCCTGCACAC10400.3376502787238118No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC8950.2905740379402034No Hit
TCGACATAGTGTGGTGGTGGTTGTCAGTGGCCTGTAGAACCATGTCGTCA8620.27986013486531325No Hit
CAATGGTTCACTGAAGACCCATGTCCAGATGAAGCTCCCAGAATGCCAGA6730.2184986899818513No Hit
CAATGGTTCACTGAAGACCCATGTCATGTAGAACCATGTCGTCAGTGTCT6330.20551214080016625No Hit
CAATGGTTCACTGAAGACCCACCAAGAAGACATAATTTATAGACAAGAGG4550.14772199694166765No Hit
CAATGGTTCACTGAAGACCCACCCCCATGATTCAATTACCTCCCACCAGG4110.1334367928418141No Hit
CAATGGTTCACTGAAGACCCACAAAGAGATTGGTACCTCAACACCCAATA3890.1262941907918873No Hit
CAATGGTTCACTGAAGACCCATGTCGTCAGTGTCTCCTGGCCCCTGTCAT3630.11785293382379201No Hit
TGACTGCTCTTTTCACCCATCTACGGTAGCAGATGAAGCTCCCAGAATGC3540.11493096025791286No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA3530.11460629652837073No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG3440.1116843229624916No Hit
CAATGGTTCACTGAAGACCCAACCTCCTGGCCCCTGTCATGTAGAACCAT3260.10584037583073333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)