FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213380
Sequences flagged as poor quality0
Sequence length125
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG118305.5440997281844595No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT90284.230949479801294No Hit
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT89844.210328990533321No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA84233.94741775236667No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA81303.810104039741306No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT80603.7772987159058955No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA75483.537351204424032No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG68233.1975817789858465No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA66943.137126253632018No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC66753.1282219514481207No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA65633.075733433311463No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC64043.0012184834567437No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC60702.844690224013497No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC60262.8240697347455246No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC59772.801106008060737No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT57462.6928484394038805No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC56612.653013403318024No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA55952.622082669416065No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA51952.4346236760708595No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA45792.1459368263192427No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGCAGGTTGAGGGCTGCTCAG42171.9762864373418316No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT39011.828193832599119No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG24211.1345955572218578No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG17410.815915268535008No Hit
CAATGGTTCACTGAAGACCCATGTCGTCAGTGTCTCCTGGCCCCTGTCAT9240.43303027462742527No Hit
CAATGGTTCACTGAAGACCCAAACTCCTGGCCCCTGTCATGTAGAACCAT8840.4142843752929047No Hit
TGACTGCTCTTTTCACCCATCTTACGGTAGCAGATGAAGCTCCCAGAATG8130.38101040397413066No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC4920.23057456181460306No Hit
CAATGGTTCACTGAAGACCCAGGTCAGGAGCCACTTGCCACCCTGCACAC4300.2015184178460962No Hit
CAATGGTTCACTGAAGACCCATGTCCAGATGAAGCTCCCAGAATGCCAGA3780.17714874871121944No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC3580.16777579904395915No Hit
CAATGGTTCACTGAAGACCCACACTGCCCTCCTGGCCCCTGTCATGTAGA3380.15840284937669885No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA2620.12278564064110975No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG2570.12044240322429467No Hit
CAATGGTTCACTGAAGACCCAGGGGATGATTGGAGGGAGGTTCTTTTTGG2180.10216515137313711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)