FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216809
Sequences flagged as poor quality0
Sequence length125
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG103594.777938185222938No Hit
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT90904.192630379735158No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT89304.118832705284374No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA88384.076399042475174No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA88234.069480510495413No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT81983.781208344672039No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG80043.691728664400463No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA76153.512308068391995No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC70183.2369504955975077No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA69603.2101988386090983No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC69153.1894432426698156No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC68633.1654589984733104No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC68193.145164637999345No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA67073.0935062658837964No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC66223.0543012513318173No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC65653.028010829808726No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT64822.9897282861873817No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT54332.50589228306943No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA49272.2725071376188257No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA48832.25221277714486No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC45692.107384841035197No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA44702.0617225299687743No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG28701.3237457854609356No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG26101.2038245644784118No Hit
TGACTGCTCTTTTCACCCATCTTACGGTAGCAGATGAAGCTCCCAGAATG13740.6337375293461065No Hit
CAATGGTTCACTGAAGACCCAAACTCCTGGCCCCTGTCATGTAGAACCAT12960.5977611630513494No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCTGCCCTCAACAAGATG12510.5770055671120664No Hit
CAATGGTTCACTGAAGACCCATGTCGTCAGTGTCTCCTGGCCCCTGTCAT9450.43586751472494223No Hit
CAATGGTTCACTGAAGACCCAGGTCAGGAGCCACTTGCCACCCTGCACAC8920.41142203506312003No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC6440.2970356396644051No Hit
TCGACATAGTGTGGTGGTGGTTGTCAGTGGCCTGTAGAACCATGTCGTCA5390.24860591580607816No Hit
CAATGGTTCACTGAAGACCCATGTCCAGATGAAGCTCCCAGAATGCCAGA4030.18587789252291187No Hit
CAATGGTTCACTGAAGACCCATGTCGTCAGTGTACTCCTGGCCCCTGTCA2550.11761504365593681No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA2530.11669257272530201No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG2440.11254145353744541No Hit
CAATGGTTCACTGAAGACCCAGGCTGGCCTCAAACTCCTGGCCCCTGTCA2290.10562292155768441No Hit
CCTCTGATTCCTCACTGAGTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT2240.10331674423109741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)