FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98598
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACG63266.415951642021136No Hit
ATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAG50285.099494918761029No Hit
GTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCT48874.956489989654963No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCA44814.544716931377919No Hit
AGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTT40914.149171382786669No Hit
ACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCT39854.041664131118279No Hit
ATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCA38403.894602324590762No Hit
GGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG37863.839834479401205No Hit
AAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAGGTC35553.605549808312542No Hit
CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCT32533.299255562993164No Hit
CATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTGGTGTTGTTGGGC32273.272885859753748No Hit
CCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAA32263.2718716403983854No Hit
GGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACA31853.230288646828536No Hit
CAAGTGGCTCCTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGG27862.825615124039027No Hit
CTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTG27342.772875717560194No Hit
GGCCACTGACAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAA22952.327633420556198No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCT22712.3032921560275055No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC21292.1592730075660764No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC20922.121746891417676No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGG17761.8012535751232277No Hit
GCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCG17251.7495283879997565No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTC15781.6004381427615164No Hit
TGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTG5480.5557922067384734No Hit
AGGAAGGGGCTGAGGTCAGTGTTCGGAACATCTCGAAGCGCAGACCAAGT4400.4462565163593582No Hit
TGACAGGGGCCAGGAGTTTGGGTCTTCAGTGAACCATTGAGACCAAGTCT4340.44017120022718514No Hit
CGCTTCTTGTCCTGCTTGCTACACTGACGACATGGTTCTACACGCTTCTT2820.28600985821213415No Hit
GCATTCTGGGAGCTTCATCTGCTACCGTAAGATGGGTGAAAAGAGCAGTC2520.2555832775512688No Hit
GCCCTTCCAATGGATCCACTACACTGACGACATGGCAGTGACCCGGAAGA2380.2413842065761983No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGTTCTACATGACATGGGTCT2280.23124201302257655No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGGTCTTCAGTGAACCATTGA2230.22617091624576566No Hit
AGGAAGGGGCTGAGGTCATTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC1990.20182965171707334No Hit
AAACGGCATTTTGAGTGTTAGACCAAGTCTCTGCTACCGTAGTGCGTGTG1500.15213290330432666No Hit
GCCCTTCCAATGGATCCACTACACTGACGACATGGTTCTACAGCCCTTCC1330.13489117426316963No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGT1310.13286273555244527No Hit
CCGTAGCTGCCCTGGTAGGAGCTAGACCAAGTCTGCTACCGTACGCCGGT1310.13286273555244527No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTTTTCAGTGAACCATTGTTCA1210.1227205419988235No Hit
ATTCTCTTCCTCTGTGCGCCTGTAGTCCCAGCTACTCAGGATAGGAAAAG1140.11562100651128827No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGTAAGTCACAGACTTGGCT1060.10750725166839084No Hit
TTGAGTTCCAAGGCCTCATTCAGCTCTCAGAACATCTCGAAGCGCTCACG1010.10243615489157994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)