FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98598
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT61696.256719203229275No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT49975.068054118744802No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC45604.62484026045153No Hit
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG40884.146128724720582No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT40694.126858556968701No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA39984.054848982737987No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA35263.5761374470070386No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC33863.4341467372563335No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA30463.0893121564331936No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA29512.9929613176737866No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA27832.8225724659729408No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC27372.7759183756262806No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT26722.7099941175277387No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA24882.523377756141098No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC23882.4219558206048806No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG20272.055822633319134No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC19481.9756993042455224No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA19251.9523722590721924No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC19081.9351305300310353No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT13641.3833952007140105No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC11581.1744660135094018No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG8220.8336883101077102No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG6780.6876407229355566No Hit
CAATGGTTCACTGAAGACCCAAACTCCTGGCCCCTGTCATGTAGAACCAT4370.44321385829327165No Hit
GCGCTTCGAGATGTTCCGAACACTGACCTCAGCCCCTTCCTTGTAGAACC4180.42394369054139025No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA3970.4026450840787846No Hit
CTTCCGGGTCACTGCCATGTCGTCAGTGTAGTGGATCCATTGGAAGGGCT3030.3073084646747398No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC2950.29919470983184243No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAAAAGAAACCACTGGATGGAGAA2850.28905251627822065No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG2360.23935576786547394No Hit
TGACTGCTCTTTTCACCCATCTTACGGTAGCAGATGAAGCTCCCAGAATG2340.23732732915474958No Hit
CAATGGTTCACTGAAGACCCATGTCATGTAGAACCATGTCGTCAGTGTCT2260.2292135743118522No Hit
CAATGGTTCACTGAAGACCCATGTCGTCAGTGTCTCCTGGCCCCTGTCAT2210.2241424775350413No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA1810.18357370332055417No Hit
CGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC1700.17241729041157022No Hit
CTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA1670.16937463234548367No Hit
CTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA1450.14706180652751577No Hit
AACACTCAAAATGCCGTTTTGTAGAACCATGTCGTCAGTGTAGATCTCGG1360.13793383232925616No Hit
CGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA1230.12474898070954786No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGAAACTCAAGGATG1190.12069210328809915No Hit
GCGCTTCGAGATGTTCCGAGAGCTAAATGAGGCCTTGGAACTCAAGGATG1060.10750725166839084No Hit
CCTCACCATCATCACACTGGAAGACTCTAGGTCAGGAGCCACTTGCCACC1000.10142193553621777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)