FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149755
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACG85505.709325231210978No Hit
GTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCT82665.519682147507596No Hit
ATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAG76255.091649694501019No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCA74074.946078595038563No Hit
AAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAGGTC66464.437915261593937No Hit
ATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCA59753.989850088477847No Hit
ACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCT59323.9611365229875464No Hit
AGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTT56683.784848586023839No Hit
GGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG53613.5798470835698306No Hit
CATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTGGTGTTGTTGGGC50463.3695035224199525No Hit
GGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACA48293.2246001802944813No Hit
CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCT45493.0376281259390336No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC45343.0276117658842776No Hit
CAAGTGGCTCCTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGG44832.993556141698107No Hit
CTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTG43922.9327902240325865No Hit
CCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAA41762.7885546392440985No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC36942.4666956028179357No Hit
GCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCG32742.1862375212847653No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCT29711.983907048178692No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGG28701.9164635571433342No Hit
GGCCACTGACAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAA28251.8864144769790658No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTC23351.5592133818570333No Hit
TGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTG21521.4370137891890087No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGGTCTTCAGTGAACCATTGA1530.10216687255851224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)