FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149755
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT82545.511669059463791No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT78535.243898367333311No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC69274.6255550732863675No Hit
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG68584.57947981703449No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA59393.9658108243464323No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT58363.8970318186371076No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA54993.6719975960735867No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA52023.473673666989416No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC51853.462321792260693No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA45923.0663416914293347No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG45323.02627625121031No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT41592.777202764515375No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA41482.769857433808554No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA40312.691729825381456No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC40302.691062068044473No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC38132.546158725919001No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC37882.529464792494408No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC25601.7094587826783747No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA24381.6279923875663584No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT21881.4610530533204233No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC20621.376915628860472No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA20131.3441955193482689No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG15801.0550565924343094No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG11740.7839471136189109No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC11080.739875129377984No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA4860.3245300657740977No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG4310.287803412239992No Hit
CAATGGTTCACTGAAGACCCATGTCCAGATGAAGCTCCCAGAATGCCAGA2770.184968782344496No Hit
CGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC2670.1782912089746586No Hit
CTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA2480.16560381957196757No Hit
CTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA2170.1449033421254716No Hit
CGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA1810.12086407799405696No Hit
CAATGGTTCACTGAAGACCCATGTCGTCAGTGTCTCCTGGCCCCTGTCAT1760.11752529130913826No Hit
CACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT1540.10283462989549598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)