FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120926
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT66955.536443775532144No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC55234.567256007806427No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT54554.51102327042985No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA48974.049584043133817No Hit
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG43773.6195689926070487No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT42783.5377007426029143No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA42783.5377007426029143No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA39043.2284206870317385No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG37833.1283594925822404No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA36553.0225096339910356No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC36413.010932305707623No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA33232.747961563270099No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC31282.586705919322561No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA31102.5718207829581727No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC30912.5561086945735405No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC30412.5147610935613516No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT28562.3617749698162513No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC27562.2790797677918726No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA25422.1021120354597027No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA21951.8151596844351092No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT16281.3462778889568827No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC15681.2966607677422555No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG9250.7649306187255015No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG8390.693812744984536No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC5390.44572713891140037No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA4370.36137803284653425No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG2850.23568132576947884No Hit
CGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC2140.17696773233217009No Hit
CAATGGTTCACTGAAGACCCATGTCCAGATGAAGCTCCCAGAATGCCAGA2050.16952516414997604No Hit
CTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA2010.16621735606900087No Hit
CGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA1790.14802441162363758No Hit
CTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA1750.14471660354266244No Hit
CAATGGTTCACTGAAGACCCATGTCGTCAGTGTCTCCTGGCCCCTGTCAT1640.13562013131998082No Hit
CGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT1290.10667681061144832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)