FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114774
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACG68175.939498492689982No Hit
GTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCT55564.840817606775054No Hit
ATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAG51764.509732169306637No Hit
ATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCA49804.338961785770296No Hit
AGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTT46254.029658284977434No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCA45843.993935908829526No Hit
ACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCT45623.9747678045550385No Hit
AAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAGGTC43003.746493108195236No Hit
CATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTGGTGTTGTTGGGC40203.502535417429035No Hit
GGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG38443.3491905832331366No Hit
GGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACA35223.068639238852005No Hit
CAAGTGGCTCCTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGG35153.0625402965828497No Hit
CTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTG34913.041629637374318No Hit
CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCT34653.0189764232317424No Hit
CCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAA31902.7793751198006516No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCT31042.7044452576367473No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC30452.65303988708244No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC28082.4465471273981914No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGG21741.8941572133061495No Hit
TGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTG20721.8052869116698904No Hit
GCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCG19921.7355847143081187No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTC17221.5003397982121385No Hit
GGCCACTGACAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAA14251.2415703905065607No Hit
GCATTCTGGGAGCTTCATCTGCTACCGTAAGATGGGTGAAAAGAGCAGTC4750.41385679683552024No Hit
CGCTTCTTGTCCTGCTTGCTACACTGACGACATGGTTCTACACGCTTCTT3440.29971944865561884No Hit
GCCCTTCCAATGGATCCACTACACTGACGACATGGTTCTACAGCCCTTCC2830.24657152316726783No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGGTCTTCAGTGAACCATTGA2750.23960130343109068No Hit
GGCCACTGACAACCACCACCACACTATGTCGAAGACCAAGTCTCTGCTAC2410.20997786955233763No Hit
AGGAAGGGGCTGAGGTCAGTGTTCGGAACATCTCGAAGCGCAGACCAAGT2370.20649275968424907No Hit
TTGAGTTCCAAGGCCTCATTCAGGGATAAAGTCCTTTACCTTCTTTTCTT2090.1820969906076289No Hit
ATCCAAATACTCCACACGCAAATACACTGACGACATGGTTCTACAGCAAT1300.11326607071287924No Hit
GCATTCTGGGAGCTTCATCTGGACCTAGGTCTTCAGTGAACCATTGTTCA1260.10978096084479064No Hit
GCCCTTCCAATGGATCCACTACACTGACGACATGGCAGTGACCCGGAAGA1240.10803840591074633No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTTCCCCCTGGC1210.10542457350967989No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCTTGGC1210.10542457350967989No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)