FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115614
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT63325.476845364748214No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC55984.8419741553791065No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA55694.816890687978965No Hit
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG54824.741640285778539No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT50484.36625322192814No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT43313.7460861141384263No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA42113.6422924559309426No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA39063.378483574653589No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG38313.3136125382739112No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC37793.268635286384002No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA35643.0826716487622607No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC34152.953794523154635No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA33252.875949279499023No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA32562.8162679260297194No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC31552.7289082637050877No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT30832.6666320687805976No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC27952.417527289082637No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC25362.1935059767848184No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA24812.145933883439722No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA24142.0879824242738767No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT20641.7852509211687166No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC15921.3769958655526149No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG13401.1590291833168993No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC11961.034476793467919No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG10400.8995450377981905No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG3580.3096510803189925No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA3580.3096510803189925No Hit
CAATGGTTCACTGAAGACCCATGTCCAGATGAAGCTCCCAGAATGCCAGA2500.2162367879322573No Hit
CGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC2010.1738543774975349No Hit
CTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA1860.16088017022159945No Hit
CGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA1590.13752659712491566No Hit
CGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT1440.12455238984898022No Hit
TGGAAGTGTCTCATGCTGGATCCTAGTGTAGAACCATGTCGTCAGTGTAG1350.11676786548341896No Hit
CTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA1240.10725344681439965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)