FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93246
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACG54545.849044463033267No Hit
GTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCT47875.133732278060185No Hit
ATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCA41924.495635201509984No Hit
ATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAG41484.448448190807112No Hit
AAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAGGTC41164.414130364841387No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCA40684.3626536258928No Hit
GGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG36603.9251013448298053No Hit
ACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCT34653.7159770928511677No Hit
CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCT34063.6527036012268623No Hit
AGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTT32823.5197220256096777No Hit
CATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTGGTGTTGTTGGGC31373.364219376702486No Hit
GGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACA29493.1626021491538507No Hit
CAAGTGGCTCCTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGG27542.953477897175214No Hit
CCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAA27432.941681144499496No Hit
CTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTG25212.703601226862278No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCT22602.4236964588293333No Hit
GGCCACTGACAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAA22382.400102953477897No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC20682.217789503034983No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGG20392.186688973253544No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC19542.0955322480320873No Hit
GCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCG15771.691225360873389No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTC15571.6697767196448106No Hit
TGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTG4690.5029706368101581No Hit
TGACAGGGGCCAGGAGTTTGGGTCTTCAGTGAACCATTGAGACCAAGTCT4470.47937713145872207No Hit
GCATTCTGGGAGCTTCATCTGCTACCGTAAGATGGGTGAAAAGAGCAGTC4100.4396971451858525No Hit
AGGAAGGGGCTGAGGTCAGTGTTCGGAACATCTCGAAGCGCAGACCAAGT3880.4161036398344165No Hit
GCCCTTCCAATGGATCCACTACACTGACGACATGGCAGTGACCCGGAAGA2570.27561503978722945No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGTTCTACATGACATGGGTCT2450.2627458550500826No Hit
TGACAGGGGCCAGGAGACACTGACGACATGGGTCTTCAGTGAACCATTGA1970.21126911610149496No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTTTTCAGTGAACCATTGTTCA1130.12118482294146665No Hit
AGGAAGGGGCTGAGGTCATTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC1060.11367779851146428No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAAGGG970.10402590995860413No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGATAGAAGATGACAGGGG940.1008086137743174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)