FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93246
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT53595.7471634171975206No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC44744.798061042832937No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT42274.533170323659997No Hit
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG37784.051648328078416No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA37073.9755056517169636No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT34253.6730798103940114No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC32663.502563112626815No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA32203.453231237801085No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA31363.3631469446410573No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA28903.0993286575295453No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA27492.9481157368680693No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC25122.693949338309418No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT25022.683225017695129No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA24522.6296034146236833No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC21572.3132359565021554No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC18772.012954979302061No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA18762.0118825472406323No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC18662.0011582266263432No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG18231.9550436479849003No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT15521.664414559337666No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC12041.2912082019604059No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG7670.8225553911159728No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG6530.7002981361130772No Hit
CAATGGTTCACTGAAGACCCAAACTCCTGGCCCCTGTCATGTAGAACCAT4640.49760847650301354No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA4200.4504214658001416No Hit
GCGCTTCGAGATGTTCCGAACACTGACCTCAGCCCCTTCCTTGTAGAACC3720.39894472685155397No Hit
TGACTGCTCTTTTCACCCATCTTACGGTAGCAGATGAAGCTCCCAGAATG3650.3914377024215516No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC2990.3206571863672436No Hit
CTTCCGGGTCACTGCCATGTCGTCAGTGTAGTGGATCCATTGGAAGGGCT2570.27561503978722945No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG2500.2681080153572271No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA2440.2616734229886537No Hit
CAATGGTTCACTGAAGACCCATGTCATGTAGAACCATGTCGTCAGTGTCT2430.2606009909272248No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAAAAGAAACCACTGGATGGAGAA2280.24451451000579114No Hit
CAATGGTTCACTGAAGACCCATGTCGTCAGTGTCTCCTGGCCCCTGTCAT2000.2144864122857817No Hit
GCGCTTCGAGATGTTCCGAGAGCTAAATGAGGCCTTGGAACTCAAGGATG1840.19732749930291915No Hit
CTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA1730.18553074662720118No Hit
CGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC1630.17480642601291208No Hit
CTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA1620.17373399395148317No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGAATGGAGAA1610.17266156189005427No Hit
CGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA1420.15228535272290503No Hit
CCTCACCATCATCACACTGGAAGACTCTAGGTCAGGAGCCACTTGCCACC1110.11903995881860883No Hit
CGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT1030.11046050232717758No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAACTACGGTTTCC980.10509834202003304No Hit
TGACTGCTCTTTTCACCCATCTACAGTCCTCCTTGCCGTCCCAAGCAATG970.10402590995860413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)