FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128493
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACG79376.176990186235827No Hit
GTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCT67105.222074354244978No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCA64635.029845983827913No Hit
ATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAG58184.527873113710474No Hit
ACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCT54864.269493279789561No Hit
ATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCA52694.100612484726794No Hit
AAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAGGTC50743.948853244923848No Hit
AGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTT50453.9262839220813586No Hit
GGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG42853.3348120131057724No Hit
CATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTGGTGTTGTTGGGC42683.321581720405003No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC42553.311464437751473No Hit
CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCT42043.271773559649164No Hit
GGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACA40103.1207925723580274No Hit
CAAGTGGCTCCTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGG37092.886538566303223No Hit
CTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTG36972.8771995361615033No Hit
CCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAA35912.794704769909645No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC33092.57523756157923No Hit
GGCCACTGACAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAA31052.416474049169994No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCT30962.4094697765637036No Hit
GCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCG29282.278723354579627No Hit
TGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTG27502.14019440747745No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGG26112.032017308335863No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTC20861.6234347396356221No Hit
GCATTCTGGGAGCTTCATCTGCTACCGTAAGATGGGTGAAAAGAGCAGTC1430.1112901091888274No Hit
TGACAGGGGCCAGGAGTACACTGACGACATGGTTCTACATGACAGGGGCC1300.10117282653529765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)