FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126788
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACG78216.168564848408367No Hit
ATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCA57124.50515821686595No Hit
GTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCT56894.487017698835852No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCA56764.4767643625579705No Hit
ATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAG53874.248824809918919No Hit
AAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAGGTC53094.187304792251632No Hit
ACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCT50173.956999085086917No Hit
AGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTT47653.7582421049310657No Hit
CATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTGGTGTTGTTGGGC44193.485345616304382No Hit
GGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG42323.3378553175379375No Hit
CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCT40013.1556614190617407No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC37372.9474398208032304No Hit
CAAGTGGCTCCTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGG37182.9324541754740197No Hit
GGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACA36722.8961731394138246No Hit
CTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTG34932.754992586049153No Hit
CCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAA33832.6682335867747735No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCT32142.5349402151623184No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC26472.0877370098116543No Hit
TGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTG26462.086948291636433No Hit
GCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCG23611.862163611698268No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGG22541.7777707669495535No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTC18101.42757989715115No Hit
GGCCACTGACAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAA16401.2934978073634729No Hit
GCCCTTCCAATGGATCCACTACACTGACGACATGGTTCTACAGCCCTTCC4780.37700728775593906No Hit
ATCCAAATACTCCACACGCAAATACACTGACGACATGGTTCTACAATCCA4340.3423036880461873No Hit
GCATTCTGGGAGCTTCATCTGCTACCGTAAGATGGGTGAAAAGAGCAGTC3980.31390983373820863No Hit
GGCCACTGACAACCACCACCACACTATGTCGAAGACCAAGTCTCTGCTAC2840.22399596176294287No Hit
ATCCAAATACTCCACACGCAAATACACTGACGACATGGTTCTACAGCAAT1610.1269836262106824No Hit
AGGAAGGGGCTGAGGTCAGTGTCGGAACATCTCGAAGCGCAGACCAAGTC1590.12540618986023913No Hit
GGAATCAACCCACAGCTGCTACACTGACGACATGGTTCTACAGGAATCAA1580.1246174716850175No Hit
TTGAGTTCCAAGGCCTCATTCACACTGACGACATGGTTCTACAGTGGGGG1400.11042054453102818No Hit
GCCCTTCCAATGGATCCACTACACTGACGACATGGCAGTGACCCGGAAGA1390.10963182635580654No Hit
TGACAGGGGCCAGGAGACTGGAGATAGAAAGCAGAAGTGCACTGGGGTGG1390.10963182635580654No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTTTTCAGTGAACCATTGTTCA1340.1056882354796984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)