FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126788
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT75395.946146322995867No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT57574.540650534750923No Hit
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG54224.276429946051677No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT48523.8268605861753477No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC47293.7298482506230872No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA47163.719594914345206No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA45703.6044420607628482No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA45063.553964097548664No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC41093.240842981985677No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA38853.0641701107360317No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA35102.7684007950279206No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG34152.693472568381866No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA33272.6240653689623623No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT32182.538095087863205No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC31842.511278669905669No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC29872.3559011893870085No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC28902.27939552639051No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC27022.1311165094488436No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA22271.7564753762185696No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT17241.3597501340820897No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC16101.269836262106824No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA14061.1089377543616115No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG10620.8376187020853708No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG9960.7855633025207432No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC8380.6609458308357258No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA4140.3265293245417547No Hit
CAATGGTTCACTGAAGACCCATGTCCAGATGAAGCTCCCAGAATGCCAGA3720.29340316118244625No Hit
TGACTGCTCTTTTCACCCATCTTACGGTAGCAGATGAAGCTCCCAGAATG3470.27368520680190556No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG3420.2697416159257974No Hit
TCGACATAGTGTGGTGGTGGTTGTCAGTGGCCTGTAGAACCATGTCGTCA3170.2500236615452566No Hit
CTTCCGGGTCACTGCCATGTCGTCAGTGTAGTGGATCCATTGGAAGGGCT2700.21295390730984004No Hit
CCTCTGATTCCTCACTGATTGCTGTAGAACCATGTCGTCAGTGTATTTGC2500.19717954380540745No Hit
CTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA2390.18850364387796953No Hit
CGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC2230.17588415307442345No Hit
CTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA1760.13881439883900684No Hit
CGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA1690.13329337161245544No Hit
GTACTGTGTATATACTTACTTCTCCCCCACTGTAGAACCATGTCGTCAGT1560.12304003533457424No Hit
CACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT1500.11830772628324447No Hit
GCGCTTCGAGATGTTCCGACACTGACCTCAGCCCCTTCCTTGTAGAACCA1460.11515285358235795No Hit
CAATGGTTCACTGAAGACCCACCACCCCAGTGCACTTCTGCTTTCTATCT1340.1056882354796984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)