FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124271
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACG70395.664233811589188No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCA67235.40995083325957No Hit
AAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAGGTC61194.923916279743464No Hit
GTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCT58314.692164704556976No Hit
ATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCA52164.197278528377497No Hit
ATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAG52024.186012826805932No Hit
ACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCT45283.643649765431999No Hit
AGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTT44033.543063144257309No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC42683.4344295933886424No Hit
GGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG40713.2759050784173303No Hit
CATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTGGTGTTGTTGGGC39073.143935431436135No Hit
CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCT38363.086802230608911No Hit
GGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACA35822.8824102163819396No Hit
CAAGTGGCTCCTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGG35212.8333239452486905No Hit
CTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTG34002.7359560959515896No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC33072.66111964979762No Hit
CCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAA31232.513056143428475No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCT29542.3770630316002928No Hit
GCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCG26582.1388739126586254No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGG24851.9996620289528528No Hit
TGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTG23831.9175833460743055No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTC23001.8507938296143107No Hit
GGCCACTGACAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAA22601.8186061108384097No Hit
GCCCTTCCAATGGATCCACTACACTGACGACATGGTTCTACAGCCCTTCC2310.1858840759308286No Hit
GGCCACTGACAACCACCACCACACTATGTCGAAGACCAAGTCTCTGCTAC1420.11426640165444873No Hit
CCCAGCCCAACCCTTGTCGTTACCAGAACGTTGTTTTCAGGAAGTCTGAA1330.107024164929871No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)