FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138520
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACG81215.862691308114352No Hit
GTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCT76375.513283280392723No Hit
AAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAGGTC69765.0360958706323995No Hit
ATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAG61154.414524978342477No Hit
GCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCA61074.408749639041294No Hit
ATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCA59794.316344210222351No Hit
ACTGTTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCT54293.9192896332659544No Hit
AGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGC51503.7178746751371645No Hit
GGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGG50233.6261911637308692No Hit
AGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTT49813.5958706323996537No Hit
CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCT47453.4254981230147274No Hit
CAAGTGGCTCCTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGG45213.263788622581577No Hit
CATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTGGTGTTGTTGGGC44483.211088651458273No Hit
GGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTTGGCAAAACA41252.9779093271729713No Hit
GCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTCC39642.8616806237366443No Hit
CCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAA37312.6934738665896623No Hit
CTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTG36232.615506786023679No Hit
GGCCACTGACAACCACCCTTAACCCCTCCTCCCAGAGACCCCAGTTGCAA33612.426364423909905No Hit
CCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGG33232.3989315622292806No Hit
TGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCT33142.392434305515449No Hit
GCCTCACAACCTCCGTCATGTGCTGTGACTGCTTGTAGATGGCCATGGCG33042.385215131388969No Hit
TGTCGTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTC27982.0199249205890846No Hit
TGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTG27952.0177591683511404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)