FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153007
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGTGTATATACTTACTTCTCCCCCTCCTCTGTTGCTGCAGATCCGT89405.8428699340553045No Hit
TGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG76084.972321527773239No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT72714.752070166724398No Hit
ACAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA67584.416791388629279No Hit
CTCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTC64744.231178965668237No Hit
CTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTT56443.6887201239158993No Hit
CCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACC53233.4789258007803565No Hit
TTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA52143.4076872299960135No Hit
CCTCAGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGA51973.396576627213134No Hit
GCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAACTCAAGGATG51103.339716483559576No Hit
CTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATCCTAGCGTCGAGC45772.9913664080728335No Hit
TTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA45632.9822164998986973No Hit
CTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACA44552.9116314939839354No Hit
AACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGAT43082.815557458155509No Hit
GAAGCGAAAATTCCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTC39652.5913847078891816No Hit
TGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA38992.548249426496827No Hit
TGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC36152.3626370035357858No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC34172.233231159358722No Hit
TCGACATAGTGTGGTGGTGCCCTATGAGCCGCCTGAGGTCTGGTTTGCAA31312.0463116066585187No Hit
CAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGA30591.9992549360486773No Hit
GGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGCC24101.575091335690524No Hit
AGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCAT24051.5718235113426182No Hit
AACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCCCG16691.0907997673309064No Hit
CTCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTC15711.0267504101119558No Hit
AACTGGCCAAGACCTGCTCTGTGCAGCTGTGGGTTGATTCCACACCCCCG13430.877737619847458No Hit
CCAACACCAGCTCCTCTCCCCAGCCAAAGAAGAAACCACTGGATGGAGAA5280.3450822511388368No Hit
CGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATG4170.27253655061533133No Hit
CAATGGTTCACTGAAGACCCATGTCCAGATGAAGCTCCCAGAATGCCAGA3350.21894423130967866No Hit
CTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACA2370.15489487409072789No Hit
CGGAAGTGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGAC2160.14117001182952413No Hit
CTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAGCACTGCCCAACAACA2040.13332723339455058No Hit
CGTGCCTGTCCTGGGAGAGACCGGCGCACAGAGGAAGAGAATCTCCGCAA1730.11306672243753554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)