FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217246
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA158377.2898925641899055No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA132376.093092623109286No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC126625.828415713062611No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG124885.7483221785441385No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA82033.7759038141093506No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT79533.6608268966977526No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC70193.230899533248023No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG64952.989698314353314No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG61462.829050937646723No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA60472.7834804783517306No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT55282.544580797805253No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC48402.2278891210885354No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG41531.9116577520414644No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT37481.725233145834676No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT36261.6690756101378161No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG35731.6446793036465575No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT31081.4306362372609853No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC29201.3440983953674637No Hit
TGTGCTGTGACTGCTTGTAGATGACCATGGCGCGGACGCGGGTGCCGGGC28501.3118768584922162No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA25781.1866731723483976No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG24981.1498485587766865No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG23961.1028971764727544No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAATTG18840.8672196496138018No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC17480.8046178065418926No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT16150.7433968864789225No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA15570.7166990416394318No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGATCGTCTCTAT14820.6821759664159525No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT14610.6725095053533782No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA14040.6462719681835338No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA13580.6250978153797998No Hit
ACTTCTACCGTGCCCTGAGGAAACAAGAAGCTGTGTCTACTCATTTGAAC12820.5901144324866741No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA12100.5569722802721339No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC10740.4943704372002246No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT10560.48608489914658953No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC10440.48056120711083294No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA10200.4695138230393195No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG9550.439593824512304No Hit
ATACGGCCAGGCATTGAAGTCTGATGGAAGCCAGCCCCTCAGGGCAACTG7450.3429292138865618No Hit
TCTTAAATGGCTACGACCCAGAATGGCTACCTGTTAAAGAATCATCTGGA7340.33786582952045147No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAAGGCAACTG7290.3355642911722195No Hit
GCAGCTTCTGCCATCTCTCTGCTACCAGAACGTGGGAGTAGACGGAAGAC5700.2623753716984432No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC5330.24534398792152673No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG5260.24212183423400202No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA5150.2370584498678917No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC4960.22831260414461024No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGCACATGCCGACGAGTACCTCATAG4840.22278891210885357No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAAATCTACTGTTTTCCTTTACT4790.2204873737606216No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4780.22002706609097522No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA4570.21036060502840095No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG4120.18964675989431334No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGATCGTCTCTATCTCG4010.18458337552820306No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT3450.15880614602800514No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA3020.13901291623321027No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT2680.12336245546523297No Hit
ATACGGCCAGGCATTGAAGTACACTGGCGACAGGGGCCAGGAGGAGACCA2620.12060060944735461No Hit
CAGCCTCTGGCATTCTGGTTAGCAGAGCAGTCAGAGGACCAGGAGACCAA2580.11875937876876905No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT2570.11829907109912265No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG2530.11645784042053708No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTTGGATAAGATGCTGAGG2450.11277537906336595No Hit
AGCTGCTCACCATCGCTACCGTACAGGTCTTGGCCAGTTGGAGACCAAGT2390.11001353304548761No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT2360.10863261003654842No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGATCGTCT2280.1049501486793773No Hit
ACTTCTACCGTGCCCTGACGGATGAAGAAGACATGGACGACGTGGTGGAT2280.1049501486793773No Hit
AGGACAAAATGTTTCACTTTTGGTAAATACATTCTTCATACCAGGACCAG2260.1040295333400845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)