FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217246
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC153497.0652624214024655No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG129385.955460629885015No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA124725.740957255829797No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC87424.0240096480487555No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA73383.3777376798652217No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA70183.2304392255783765No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT58692.7015457131546725No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC58522.6937204827706838No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG52082.3972823435184076No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA49912.2973955792051406No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC48492.232031890115353No Hit
AGAATGGTCCTGCACCAGTAATATGTATATTAAAACAAGATTTACCTCTA48192.2182226600259614No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT46732.151017740257588No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC39921.8375482172283952No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG38131.7551531443616912No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC36331.672297763825341No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAAAGAGACAGAGATTTCA32851.5121106947883967No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAAGTAAAGAGGAGCA32091.4771273118952708No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG26671.2276405549469267No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA25151.157673789160675No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG23551.0840245620172522No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT22441.032930410686503No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT21550.991963028087974No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA17260.794491037809672No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT15060.6932233504874658No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG14710.6771125820498421No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT14070.647652891192473No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT14050.6467322758531803No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT13770.6338436611030813No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA13500.6214153540226287No Hit
GTTCAAATGAGTAGACACAGCTTCTTGTTTCCTCAGGGCACGGTAGAAGT12390.5703212026918792No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA11670.537179050477339No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA10730.4939101295305782No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA10140.46675197702144117No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA10100.46491074634285556No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT9220.4244036714139731No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAAATTTCA8000.3682461357171133No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG7500.3452307522347937No Hit
CCTCCTGGCCCCTGTCATCTTCTGTTCCTTCCCAGAAAACCTACCAGGGC7470.3438498292258546No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTCTGGGTCGTAGCCATTTAAGA7130.3281993684578772No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC6950.3199138304042422No Hit
TCCGTCTACTCCCACGTTCTGGTAGCAGAGAGATGGCAGAAGCTGCTGTA5650.2600738333502113No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA5230.2407409112250628No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC5110.23521721918930613No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC4850.22324921977849993No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA4760.21910645075168242No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG4690.21588429706415768No Hit
ATGAGGTACTCGTCGGCATGTGCAGAGACTTGGCTATAGGAGCAGGGGTG4680.2154239893945113No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT4430.20391629765335148No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT4250.19563075959971643No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG4180.19240860591219172No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC3960.1822818371799711Illumina Single End PCR Primer 1 (100% over 21bp)
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT3800.17491691446562882No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA3730.17169476077810408No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG3500.1611076843762371No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCGAATGCTATC3150.14499691593861336No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA2990.1376319932242711No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAAAGTG2770.12750522449205048No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA2770.12750522449205048No Hit
CCTCCTGGCCCCTGTCGCCAGTGTACTTCAATGCCTGGCCGTATTGTAGA2730.1256639938134649No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCTAGAGGCTGCTCCCCCG2650.12198153245629378No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT2640.12152122478664738No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT2550.11737845575982987No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT2530.11645784042053708No Hit
CCTGGTCCTCTGACTGCTCTGCTAACCAGAATGCCAGAGGCTGTGTAGAA2490.11461660974195152No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG2460.11323568673301235No Hit
CGAATGGTCCTGCACCAGTAATATGTATATTAAAACAAGATTTACCTCTA2450.11277537906336595No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGACAGTAAGATACAGTCTA2430.11185476372407316No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT2350.10817230236690205No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG2320.10679137935796286No Hit
CCAACTGGCCAAGACCTGTACGGTAGCGATGGTGAGCAGCTTGTAGAACC2320.10679137935796286No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCCTGCCTCT2290.10541045634902368No Hit
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA2190.10080737965255976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)