FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145270
Sequences flagged as poor quality0
Sequence length150
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT1572310.823294554966614No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA1536010.573415020307014No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA89616.168513801886143No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT72044.959041784263785No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT70934.882632339781097No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG45433.127280236800441No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA44163.039856818338267No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC42782.9448612927651956No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA30792.1195016176774284No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA28951.9928409169133336No Hit
AGAAAACGGCATTTTGAGTGTAGTGGTGATGGTGGTGGTGGTGTGATGGT24241.6686170578922008No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA22611.5564121979761822No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCCGCCCCTCAGGGCAACTG21521.4813795002409307No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGCTGTAGAACAGGGACGAACT20191.3898258415364493No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC19781.3616025332140151No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTGCTCATGTAT19511.3430164521236319No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC18741.2900117023473532No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG18571.2783093549941489No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA17971.2370069525710745No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA13110.9024574929441729No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG12050.829489915330075No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG11790.8115922076134094No Hit
CAGCCTCTGGCATTCTGCTACCGTACAGAGCAGTCAGAGGACCAGGAGAC11050.7606525779582846No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC10980.7558339643422592No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG10760.7406897501204653No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTAGCTACCTGTTAAAGAATCAT9660.6649686790114958No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG9270.6381221174364976No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT7680.5286707510153507No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC6360.4378054656845873No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG5900.4061402904935637No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC5860.40338679699869207No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG5500.37860535554484753No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT4830.3324843395057479No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG4800.3304192193845942No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTGCTCATGTATCTCG4420.3042610311833138No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG3440.23680044055895919No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACGTAGCTAATGA3210.2209678529634474No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT3040.209265505610243No Hit
ATACGGCCAGGCATTGAAGTTACACTGACGACAGGGGCCAGGAGGAGACC2800.1927445446410133No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTG2770.19067942451985956No Hit
TGTGCTGTGACTGCTTGTAGGTGGCCATGGCGCGGACGCGGGTGCCGGGC2690.18517243753011634No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA2480.17071659668204034No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTGCTCAT2480.17071659668204034No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC2270.15626075583396434No Hit
GGAATCCTATGGCTTTCCAACCTACGGCAGCAACAGAGGAGGGGGAGACC2010.13836304811729883No Hit
AGGACAAAATGTTTCACTTTTGGTAAATACATTCTTCATACCAGGACCAG1980.1362979279961451No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC1960.1349211812487093No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTAGTGCTCA1940.13354443450127348No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGGGAACGCCTCCCT1720.11840022027947959No Hit
AGCTGCTCACCATCGCTACCGTACAGGTCTTGGCCAGTTGGAGACCAAGT1710.11771184690576167No Hit
AGGACAAAATGTTTCACTTTGGGTAAATACATTCTTCATACCAGGACCAG1700.11702347353204379No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT1650.11358160666345425No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTGCTAATGAGTTTGTACTGAG1460.10050251256281408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)