FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246078
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT178607.25786132852185No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA169056.8697729988052565No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG138955.646583603572851No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG135885.521826412763433No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC135785.517762660619804No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA128685.229236258422127No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT75013.0482204829363044No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT74323.020180593145263No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA70012.845032875754842No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC67992.7629450824535313No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG59762.4284982810328435No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC54172.201334536203968No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG49252.001397930737409No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT45041.8303139654906166No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT40081.6287518591666057No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA39981.6246881070229764No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG32971.3398190817545657No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG30161.2256276465185836No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG23840.9687985110412146No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACAGTCAGAGTAT18630.7570770243581304No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT17570.7140012516356603No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA16360.6648298506977462No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA15950.6481684669088663No Hit
GCCTGCTGAAAATGACTGAATATAAATTTGTGGTAGTTGGAGCTGGTGGC15450.62784970619072No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC15210.6180967010460098No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC14180.5762400539666285No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTAGTGGGGGTTGT14070.5717699266086362No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA14060.5713635513942734No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCGCTTGATAAGAG13680.5559212932484822No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGCTGCCGGGC12790.5197538991701818No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAATAATT12050.4896821333073253No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT10200.41450271865018407No Hit
GCAGCTTCTGCCATCTCTCTGCTACCCGTAGAACGTGGGAGTAGACGGAA9940.403936963076748No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG9870.4010923365762076No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG8960.36411219206918133No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG8950.3637058168548184No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC8610.3498890595664789No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG8590.34907630913775306No Hit
GGAATCCTATGGCTTTCCAACCCAGCAACAGAGGAGGGGGAGACCAAGTC8510.34582530742284967No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA7130.28974552784076596No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG7000.2844626500540479No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTAGTGCTAAAATGGAGATTC6340.2576418859060948No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA5580.22675736961451248No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTCGGAGCTGGTGGC5490.22309999268524616No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACAGTCAGAGTATCTCG4670.18977722510748624No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGAGATAACACAGGCCCAAGA4620.1877453490356716No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT4500.1828688464633165No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAGAGACATTATGACA4230.1718967156755175No Hit
CAGAGGGGAAAAATATGACAAATACGGTGCTAAAATGGAGATTCTCTGTT3800.1544225814579117No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC3610.14670145238501614No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT3440.1397930737408464No Hit
CAGCCTCTGGCATTCTGGCCGTAAGAGCAGTCAGAGGACCAGGAGACCAA3440.1397930737408464No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT3380.13735482245466885No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA3370.13694844724030591No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATGCCAGGACCA3270.13288469509667666No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA3260.13247831988231373No Hit
TCTTAAATGGCTACGACCCAGAACCCAAAATGGCTACCTGTTAAAGAATC3060.12435081559505523No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC3060.12435081559505523No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC2970.12069343866578888No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACAG2800.11378506002161916No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACAGTCAGA2500.10159380359073139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)