FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323392
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT212316.565097466851376No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA168385.206684148030873No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA159484.931476350682763No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG155494.80809667524243No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC152794.724606669305363No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG122753.795703047694439No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA120953.740043043736394No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA117943.6469671482287747No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT108103.3426924599247974No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG108003.3396002374826836No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT100783.1163417771620816No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA98213.0368716603997625No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC89182.7576439738769047No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGAATAAGACGCTGAGG72142.230729269740748No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT69412.1463115970710467No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG56411.7443226795962794No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT52061.6098110033643378No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT46101.4255145458143679No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG36131.1172199683356423No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA34311.0609415198891747No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCATGCGCGAAT27360.8460320601622798No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA25600.7916089451810805No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA22650.7003883831387294No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGGGCGGACGCGGGTGCCGGGC19640.6073124876311102No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT17290.5346452602414408No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC17090.5284608153572136No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCGGCCCCTCAGGGCAACTG15510.47960370077181874No Hit
AGCTGCTCACCATCGCTACGACATGGTTCTACAGGTCTTGGCCAGTTGGA14790.45733969918860085No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC14610.4517736987927964No Hit
AGAAAACGGCATTTTGAGTGTACTGAGGCATAACTGCACCCTTAGACCAA13700.4236344745695626No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG13550.41899614090639226No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC12410.3837448050662972No Hit
TCCCTGCCAGCGAGATGTACACTGAGGCCTTTGCATCCCTAGACCAAGTC11140.34447358005145456No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCTGGGC10780.33334157925984564No Hit
GGGGTCAGAGGCAAGCATTATGATGTAGAAGGAGATAACACAGGCCCAAG10330.31942657827033444No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG9480.2931426875123689No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC8760.270878685929151No Hit
GGTCTTCTCTGTTTCAGGGCATGACCAAGTCTCTGCTACCGGGAGGTGGC8450.26129279635859887No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG7740.23933801701959234No Hit
TCCACAAACAGAACAAGATGCTTCTACCGTAATCCTGTTTGAAGGAAAAG7400.22882446071640608No Hit
TGACAGTTTGACAGTTAAAGGCAAAGATGTATTCCTCCAATTCAGGACCC7070.21862012665743122No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT7030.2173832376805858No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC6680.20656045913318818No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTTTGCAAATATCTTCTAAAACAA6650.2056327924005541No Hit
TGACAGTTTGACAGTTAAAGGCATAGATGTATTCCTCCAATTCAGGACCC6450.19944834751632695No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCATGCGCGAATCTCG6320.19542845834157924No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5970.1846056797941817No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT5650.17471056797941817No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC5610.17347367900257274No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC5590.17285523451415002No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT5300.16388778943202056No Hit
GGAATCCTATGGCTTTCCAACCGTAACACTGACGACATGGTTCTACAGCA5120.1583217890362161No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT4900.15151889966356621No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTTCATCTGGACCTGG4710.14564367702355036No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTATCATGC4710.14564367702355036No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT4430.1369854541856323No Hit
AGCTGCTCACCATCGCTACCCGTAAGGTCTTGGCCAGTTGGAGACCAAGT3840.11874134177716208No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATCATGCGC3650.11286611913714625No Hit
ATACGGCCAGGCATTGAAGTCCCAGAGAAAGAGGACAGGGGCCAGGAGGA3500.10822778547397585No Hit
TGACAGTTTGACAGTTAAAGGCAAAGATATATTCCTCCAATTCAGGACCC3500.10822778547397585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)