FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323392
Sequences flagged as poor quality0
Sequence length150
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC240857.447617751830596No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG228687.071294280625372No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA153574.748726004353849No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA127073.929287057193746No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC121513.7573594894122304No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT116833.6126434791213144No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG106783.301875123688898No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA106033.278683455373046No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA97883.0266673263407875No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT97743.0223382149218287No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC96072.9706981001385317No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG87982.7205373045715415No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC68422.1156985948941225No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG54561.687116564417178No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA50351.5569339996041955No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT46161.4273698792796359No Hit
GCCTCAAAGACAATGGCTCCTGGTTATAGCTAACTAACTTCAGAACACCA40361.2480209776370472No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA34081.0538294082723134No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT33611.0392959627943796No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG27450.8488150603601821No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA24710.7640881654462696No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT22430.6935854937660796No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC20080.6209182663764101No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA16700.5164011478329705No Hit
GGCCTCAGTACAAACTCATTAGCTAGCAGAGACTTGGTCTGGACCTAATT16570.5123812586582228No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTAGCGATGGTGAGCAGCTT14370.44435236493172375No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA14170.4381679200474965No Hit
CCTCCTGGCCCCTGTCATCTTCTGCCCCTTCCCAGAAAACCTACCAGGGC14010.43322036414011483No Hit
AAGGGTGCAGTTATGCCTCAGTACACTCAAAATGCCGTTTTCTTGTAGAA13510.41775925192954677No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG13260.4100286958242628No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT12630.39054769443894716No Hit
AGGGATGCAAAGGCCTCAGTGTACATCTCGCTGGCAGGGATGTAGAACCA12500.3865278052641995No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC12040.3723035820304769No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC11590.35838858104096577No Hit
CTTGGGCCTGTGTTATCTCCTTCTACATCATAATGCTTGCCTCTGACCCC10120.31293291114189586No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC9490.29345190975658025No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG9210.2847936869186622No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC8870.27428013061547596No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA7710.23841035028695826No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT7630.23593657233326737No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA7610.23531812784484463No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC7490.2316074609143083No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGAAGCATCTTGTTCTGTTTGT7220.22325846032060162No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT6960.21521868197110627No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA6530.20192212547001778No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGGGAGAGAGAGACAGAGATTTCA6480.200376014248961No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG6460.1997575697605383No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC6360.1966653473184247Illumina Single End PCR Primer 1 (100% over 21bp)
AATAGTTGTTTTAGAAGATATTTGCAAACTATCAAGAAAAACCAAAAGAC6250.19326390263209975No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG5940.1836780130615476No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTTACGGTTGGAAA5790.1790396793983772No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG5610.17347367900257274No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA5410.16728923411834554No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT5240.16203245596675242No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG4930.15244656639620027No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA4920.1521373441519889No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT4600.1422422323372254No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT4590.14193301009301404No Hit
AAGACCCAGGTCCAGATGAAGACTTGGTCTAAGACCCAGGGCAGCTACGG4500.1391500098951118No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGTGCCTCTG4330.1338932317435187No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT4230.1308010093014051No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA3870.11966900850979617No Hit
CCAACTGGCCAAGACCTTACGGGTAGCGATGGTGAGCAGCTTGTAGAACC3740.11564911933504848No Hit
CCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT3730.11533989709083713No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT3580.11070156342766672No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG3550.10977389669503265No Hit
CCTCCTGGCCCCTGTCCTCTTTCTCTGGGACTTCAATGCCTGGCCGTATT3500.10822778547397585No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGCGAATTGCTGCAACA3490.1079185632297645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)