FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4804
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA145430.266444629475437No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGCT51910.803497085761865No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGCTCATATGCAT4128.576186511240632No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT2084.329725228975854No Hit
ATACGGCCAGGCATTGAAGTTACACTGACGACAGGGGCCAGGAGGAGACC1332.768526228143214No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG1272.643630308076603No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGCTCATATGCATCT1122.3313905079100747No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT1032.144046627810158No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG811.6860949208992508No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG731.5195670274771023No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT681.4154870940882598No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG480.9991673605328892No Hit
GGGGTCGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGC410.8534554537885095No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAGCT390.8118234804329725No Hit
GGAAACCGTAGCTGACCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGCT340.7077435470441299No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTAGCT280.5828476269775187No Hit
GGAAACCGTAGCTGCCCTGGGTCTGAGACCAAGTCTCTGCTACCGTAGCT220.4579517069109076No Hit
TCTGTATCAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGC220.4579517069109076No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA200.4163197335553705No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC170.35387177352206495No Hit
GCCTGCTGAAAATTACTGGTGCAGGACCATTCTAGACCAAGTCTCTGCTA160.33305578684429643No Hit
GGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTAGCTCATATGCATCT140.29142381348875934No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAGCT130.2706078268109909No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT120.2497918401332223No Hit
GCGTGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAGCTCA120.2497918401332223No Hit
TCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA110.2289758534554538No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGAGACCAAGTCTCTGCTACCGTAG110.2289758534554538No Hit
GCAACAGGGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA100.20815986677768525No Hit
GGAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGCTCATATGCAT100.20815986677768525No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGCTCATATGCATCTCG90.18734388009991673No Hit
GCAACGGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA90.18734388009991673No Hit
GGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGCTCAT80.16652789342214822No Hit
TCATAATGCTTGCTCTGATGGAGTGGGTCCCATCAGAGACCAAGTCTCTG80.16652789342214822No Hit
NCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA80.16652789342214822No Hit
GAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGCTC80.16652789342214822No Hit
GGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGCTCATA70.14571190674437967No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGACCAAGTCTCTGCTACCGTAGCT70.14571190674437967No Hit
GCAACAGAGGGGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA70.14571190674437967No Hit
GGAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA70.14571190674437967No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT60.12489592006661115No Hit
GCGACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA60.12489592006661115No Hit
GGAAACCGTAGCTGCCCTGTGTCTTAGACCAAGTCTCTGCTACCGTAGCT60.12489592006661115No Hit
GCAACAGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTAGCT60.12489592006661115No Hit
GGGGTCAGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGC60.12489592006661115No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAAACCAAGTCTCTGCTACCGTA60.12489592006661115No Hit
CAGAGGGGGGGGAGACCAAGTCTCTGCTACCGTAGCTCATATGCATCTCG60.12489592006661115No Hit
GGGGTCAGGAGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCG60.12489592006661115No Hit
GTAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA60.12489592006661115No Hit
GCAGCAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA50.10407993338884262No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGCTCATATGCAG50.10407993338884262No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGC50.10407993338884262No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACAAGTCTCTGCTACCGTAG50.10407993338884262No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT50.10407993338884262No Hit
TGCTGCATATTTCAGATATTCATGTTGTGTTTTCATAGACCAAGTCTCTG50.10407993338884262No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGCTCATATGCAT50.10407993338884262No Hit
GCAACAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTAGC50.10407993338884262No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA50.10407993338884262No Hit
GCCTGCTGAAAATGACTGGTGCAGGACCATTCTAGACCAAGTCTCTGCTA50.10407993338884262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)