FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4804
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT144029.97502081598668No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA50610.532889258950874No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG4228.784346378018318No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG2064.288093255620316No Hit
CCTCCTGGCCCCTGTCGTCAGTGTAACTTCAATGCCTGGCCGTATTGTAG1342.7893422148209823No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC1152.3938384679433806No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA1132.3522064945878434No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT1022.12323064113239No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT851.7693588676103247No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA711.4779350541215655No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT711.4779350541215655No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG430.8950874271440467No Hit
CTTGGGCCTGTGTTATCTCCGACCCCTGTAGAACCATGTCGTCAGTGTAG390.8118234804329725No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA390.8118234804329725No Hit
AAGACCCAGGTCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA340.7077435470441299No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA280.5828476269775187No Hit
GCCTCAAAGACAATGGCTGATACAGATGTAGAACCATGTCGTCAGTGTAG220.4579517069109076No Hit
CAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA210.43713572023313907No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA200.4163197335553705No Hit
AGAATGGTCCTGCACCAGTAATTTTCAGCAGGCTGTAGAACCATGTCGTC170.35387177352206495No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC160.33305578684429643No Hit
GTTTCCTCTGGTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC150.31223980016652786No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA130.2706078268109909No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT130.2706078268109909No Hit
GGCTCCTTATCTCCCCTCCACGCTGTAGAACCATGTCGTCAGTGTAGATC120.2497918401332223No Hit
CCCCCTCCTCTGTTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG110.2289758534554538No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGT110.2289758534554538No Hit
CTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA110.2289758534554538No Hit
GCTGACCTAAAGCCACCTCCCCTGTTGCTGTAGAACCATGTCGTCAGTGT100.20815986677768525No Hit
CTGATGGGACCCACTCCATCAGAGCAAGCATTATGATGTAGAACCATGTC90.18734388009991673No Hit
GCTGACCTAAAGCCACCTCCTCCGTTGCTGTAGAACCATGTCGTCAGTGT90.18734388009991673No Hit
CTTGGGCCTGTGTTATCTCCCCTGTAGAACCATGTCGTCAGTGTAGATCT80.16652789342214822No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTATAGAACCATGTCGTCAGTGT80.16652789342214822No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA80.16652789342214822No Hit
GCTGACCTAAAGCCACCTCCTCTGTTCCTGTAGAACCATGTCGTCAGTGT70.14571190674437967No Hit
GCTGACCTAAAGCCACCCCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT70.14571190674437967No Hit
AAGACCCAGGGCAGCTACGGTTTCTGTAGAACCATGTCGTCAGTGTAGAT70.14571190674437967No Hit
GACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA70.14571190674437967No Hit
GCTGACCTAAAGCCACCTCCTCTGTTACTGTAGAACCATGTCGTCAGTGT70.14571190674437967No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC70.14571190674437967Illumina Single End PCR Primer 1 (100% over 21bp)
GCTGACCTAAAGCCACCTCCTGTTGCTGTAGAACCATGTCGTCAGTGTAG60.12489592006661115No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG60.12489592006661115No Hit
CTTGGGCCTGTGTTATCTCCTCCTGACCCCTGTAGAACCATGTCGTCAGT60.12489592006661115No Hit
CTTGGGCCTGTGTTATCTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC60.12489592006661115No Hit
CTTGGGCCTGTGTTATCTCTGACCCCTGTAGAACCATGTCGTCAGTGTAG60.12489592006661115No Hit
GTTTCATCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC60.12489592006661115No Hit
CCCCCCCCTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC60.12489592006661115Illumina Single End PCR Primer 1 (95% over 22bp)
AAGACACAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA60.12489592006661115No Hit
GCTGACCTAAAGCCACCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA60.12489592006661115No Hit
CCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT50.10407993338884262No Hit
GCTGACCTAAAGCCACCTCCTCTGTCGCTGTAGAACCATGTCGTCAGTGT50.10407993338884262No Hit
GCTGACCTAAAGCCACCTCCTCTGCTGCTGTAGAACCATGTCGTCAGTGT50.10407993338884262No Hit
ATGAAAACACAACATGAATATCTGAAATATGCAGCATGTAGAACCATGTC50.10407993338884262No Hit
AGAATGGTCCTGCACCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC50.10407993338884262No Hit
GCCTCAAAGACAATGGCTCTTGATACAGATGTAGAACCATGTCGTCAGTG50.10407993338884262No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG50.10407993338884262No Hit
CCCCCTCCTCTGCTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG50.10407993338884262No Hit
GCTGACCTAAAGCCACCTCTTCTGTTGCTGTAGAACCATGTCGTCAGTGT50.10407993338884262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)