FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82860
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA2194126.479604151580983No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC922311.130823075066377No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT51096.165821868211441No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT33914.09244508810041No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG27743.3478155925657735No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA22712.7407675597393193No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA17382.0975138788317644No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTAGAGTATCAT17232.079411054791214No Hit
ATACGGCCAGGCATTGAAGTTGGTTCTACAGCGACAGGGGCCAGGAGGAG16672.0118271783731596No Hit
ATACGGCCAGGCATTGAAGTTGGTTCTACAGCAACAGGGGCCAGGAGGAG10891.3142650253439536No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA10561.274438812454743No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC9001.0861694424330195No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC8681.0475500844798455No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC5660.6830798937967657No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA5020.6058411778904176No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT4920.5937726285300508No Hit
GGAAACCGTAGCTGCCCTGTTAAACTACCTGTTAAAGAATCATCTGGACC4910.5925657735940141No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC4670.5636012551291335No Hit
TGACAGTTTGACAGTTAAAGGCAAAGATGTATTCCTCCAATTCAGGACCC3490.42119237267680426No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA3450.4163649529326575No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACTA3420.4127443881245474No Hit
CAGCCTCTGGCATTCTGAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCT3260.39343470914796036No Hit
AGGACAAAATGTTTCACTTTTGGTAAATACGTTCTTCATACCAGGACCAG3230.38981414433985034No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC3170.3825730147236302No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT3070.37050446536326337No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTACTAGAG3040.3668839005551533No Hit
GGTCTTCTCTGTTTCAGGGCATGGGAGGTGGCTTTAGGTCAGCAGACCAA2860.34516051170649287No Hit
AGGACAAAATGTTTCACTTTGGGTAAATACGTTCTTCATACCAGGACCAG2790.33671252715423605No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGCTCTTCATACCAGGACCA2780.3355056722181994No Hit
TGACAGTTTGACAGTTAAAGGCGAAGATGTATTCCTCCAATTCAGGACCC2710.32705768766594256No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACTAGAGTATCATCT2370.28602461984069516No Hit
TGACAGTTTGACAGTTAAAGGCATAGATGTATTCCTCCAATTCAGGACCC2270.2739560704803283No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCGTACCAGGACCA2220.26792179580014486No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACTA2120.2558532464397779No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTCTTGGCCAGTTGG1970.2377504223992276No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC1890.2280955829109341No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG1810.21844074342264058No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT1780.21482017861453054No Hit
AGCTGCTCACCATCGCTACCGCAGGTCTTGGCCAGTTGGAGACCAAGTCT1680.20275162925416362No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC1620.1955104996379435No Hit
GGGGTCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAC1400.1689596910451364No Hit
GGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCAG1390.1677528361090997No Hit
CCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA1360.16413227130098962No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA1290.1556842867487328No Hit
GGAAACCGTAGCTGCCCTGTTAAGCTACCTGTTAAAGAATCATCTGGACC1110.13396089790007243No Hit
AGGACAAAATGTTTCACTTGGGTAAATACGTTCTTCATACCAGGACCAGA1100.13275404296403573No Hit
AGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCAG1010.12189234853970553No Hit
ACTTCTACCGTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAG1000.12068549360366883No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCCTCATACCAGGACCA970.11706492879555877No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACT960.11585807385952207No Hit
AGGACAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCAG950.1146512189234854No Hit
GCGTGGACAACCCCCACACACTGCGACATGGTTCTACAGCAACAGAGGAG940.11344436398744871No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGGGGCAAGGAAAGGTGATAAAAG910.10982379917933865No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACTAGAGTATCA910.10982379917933865No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTCCATACCAGGACCA900.10861694424330195No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTAGAGTATCAT870.1049963794351919No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACT850.10258266956311851No Hit
GCGTGGACAACCCCCACCACTGACGACATGGTTCTACAGCAACAGAGGAG850.10258266956311851No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGGCCA850.10258266956311851No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)