FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82860
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC2036424.57639391745112No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG80769.746560463432296No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA50086.043929519671735No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT33204.006758387641805No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT22572.7238715906348054No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT19282.3268163166787352No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG17382.0975138788317644No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA16822.02993000241371No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC16722.017861453053343No Hit
CCTCCTGGCCCCTGTCGCTGTAGAACCAACTTCAATGCCTGGCCGTATTG16381.9768283852280955No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC13791.6642529567945934No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT11141.3444363987448709No Hit
CCTCCTGGCCCCTGTTGCTGTAGAACCAACTTCAATGCCTGGCCGTATTG10571.2756456673907797No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA8791.0608254887762492No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC5960.7192855418778663No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGGGCACT5930.7156649770697563No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG5880.7096307023895728No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG4890.5901520637219406No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGTAGTTTAACAGGGCAGCTA4720.569635529809317No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGGGCAGCTACGGTTT4550.5491189958966932No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA4370.5273956070480328No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG4200.5068790731354091No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA3360.40550325850832725No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC3300.39826212889210716No Hit
CCTGGTCCTCTGACTGCTCTCAGAATGCCAGAGGCTGTGTAGAACCATGT3240.391020999275887No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA3130.37774559497948346No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT3050.36809075549118997No Hit
GCTGACCTAAAGCCACCTCCCATGCCCTGAAACAGAGAAGACCTGTAGAA2800.3379193820902728No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT2620.31619599324161235No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2390.2884383297127685No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC2190.2643012309920348No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA2070.2498189717595945No Hit
CCAACTGGCCAAGACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCT1960.23654356746319094No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT1740.2099927588703838No Hit
CCAACTGGCCAAGACCTGCGGTAGCGATGGTGAGCAGCTTGTAGAACCAT1660.20033791938209028No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATGCTGCAAATGCTATC1620.1955104996379435No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA1570.18947622495776006No Hit
TGCCCTGACTTTCAACTCTGTTTCCTTCCTCTTCCTACAGTACTCCCCTG1550.1870625150856867No Hit
GCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC1450.1749939657253198No Hit
CTTGGGCCTGTGTTATCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTA1380.166545981173063No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC1240.14965001206854936No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCGAATGCTATC1160.13999517258025584No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGTTATC1140.13758146270818247No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTATCCTCC1140.13758146270818247No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG1120.1351677528361091No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGT1090.13154718802799903No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGTAGCTTAACAGGGCAGCTA1040.12551291334781559No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCCCTTCCTACAGTACTCCCCTG1030.12430605841177891No Hit
ACTAGATATTCCTTGTCATTATCGCACGCTCTATACTGCAAATGCTATCG980.11827178373159547No Hit
ACTAGATATTCCTGTCATTATCTGCACGCTCTATACTGCAAATGCTATCG980.11827178373159547No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG960.11585807385952207No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG930.11223750905141201No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG900.10861694424330195No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG870.1049963794351919No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGCAGTGTGT850.10258266956311851No Hit
GCCTCAAAGACAATGGCTCTTGGTTGTAGCTAACTAACTTCAGAACACCA850.10258266956311851No Hit
CTCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT840.10137581462708181No Hit
CTTGGGCCTGTGTTATCTCCCCTCCCTGTAGAACCATGTCGTCAGTGTAG830.10016895969104514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)