FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390600
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG196515.030977982590886No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT141783.629800307219662No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA131203.3589349718381976No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA128893.2997951868919606No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT113092.8952892985151046No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG109942.8146441372247826No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT108142.768561187916027No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT92302.363031233998976No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA91602.345110087045571No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC90952.328469022017409No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC90562.318484383000512No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA81742.092677931387609No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC78812.017665130568356No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG77291.9787506400409625No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA76281.9528929851510497No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT74781.9144905273937534No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT73731.8876088069636456No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC71431.8287250384024578No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT66341.6984126984126984No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC66091.6920122887864824No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA63371.6223758320532515No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG60791.5563236047107016No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG59571.525089605734767No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT58151.4887352790578596No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG51841.327188940092166No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG51811.32642089093702No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA50641.2964669738863288No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG49691.2721454173067077No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA46561.1920122887864824No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT41671.0668202764976957No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC40911.047363031233999No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG39271.0053763440860215No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG39040.9994879672299027No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC36390.9316436251920123No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGATGGC32910.8425499231950845No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGTGGTGGGAGGCTG25710.6582181259600615No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC23390.5988223246287763No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATAGATAGC22920.5867895545314901No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT21930.5614439324116743No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC21100.5401945724526369No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG18580.4756784434203789No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTAGGAGGCTG17210.4406041986687148No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC15320.3922171018945213No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG13670.34997439836149513No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA12980.3323092677931388No Hit
TGTGGTCTGCCAGCTAAAGGTGGAGATATATTCCTCCAATTCAGGACCCA12720.32565284178187404No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGCTACTAGCGAT11030.28238607270865335No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT10510.2690732206861239No Hit
GGATGCAGAGCTTCTTCCCATGATTATCTGTCCCTCACAGCAGGGTCTTC10130.2593445980542755No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA9560.24475166410650281No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA9070.2322068612391193No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT8450.21633384536610345No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAACGCCT8180.2094214029697901No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAAG7480.19150025601638504No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGCGTGGGAGGCTG7250.18561187916026628No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACGCGGATAAGATGCTGAGG6330.1620583717357911No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC5580.14285714285714285No Hit
CAGCCTCTGGCATTCTGGGATCTTCATCTGGACCTGGGTCTTCAGTGAAC5250.13440860215053765No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTGTTCATACCAGGACCA5110.13082437275985664No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAGGCCAGCCCCTCAGGGCAACTG5090.13031233998975936No Hit
AGAAAACGGCATTTTGAGTGTTAGACTAGAAACTTTCCACTTGATAAGAG4930.12621607782898106No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCACTCATGGTGGGGGCAGCGCCT4890.1251920122887865No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA4330.11085509472606248No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG4180.10701484895033282No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA4170.10675883256528419No Hit
GACCCAAAACCCAAAATGGCAAGGGAGGGAGAGATGGGGGTGGGAGGCTG3960.10138248847926268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)