FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458356
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG214124.671478065084781No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT167553.6554555847419907No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA159563.4811369328644113No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA152723.331907949279599No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT151423.30354571555734No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG134142.9265461780799202No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC114232.492167660072084No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT108942.376755185925351No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC106022.313049245564583No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA102342.232762307027725No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA98292.144403040431455No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT98232.1430940142596584No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA96932.114731780537399No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC93152.0322631317142132No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC88301.9264501828273222No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT88051.9209959071115028No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG84071.8341638377156622No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG80011.745586400090759No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT79081.7252964944279119No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA78751.7180968504830307No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT76871.6770806971000707No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG75321.643264187661992No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG72031.571485919241812No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG69211.5099616891673722No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT69171.5090890050528412No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC65561.4303292637164127No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA63521.3858223738753284No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG61761.3474242728359616No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG61511.3419699971201424No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA60491.3197165521996002No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG57301.250119994065748No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT51421.1218354292296817No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC49281.0751468291022699No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC44530.9715155905017061No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC41410.9034462295682832No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA34800.759235179642025No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT30590.6673851765876305No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT24770.5404096379233608No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG24610.5369189014652366No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGATGGC24000.5236104687186379No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC23220.5065931284852822No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGTGAGGCTG19960.43546937315100054No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAGTTCAGGACCCA13940.3041304139140755No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA10600.23126129035073176No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTGACGTACGAT9830.2144621211460088No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAGGATGCTGAGG9050.19744478091265305No Hit
AGAAAACGGCATTTTGAGTGTTAGGCTGGAAACTTTCCACTTGATAAGAG8500.1854453743378509No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGCGAAATAATACTGGTATGTA6960.151847035928405No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG6540.14268385272582884No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCACTG6500.14181116861129778No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5710.12457565734930927No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT5460.11912138163349013No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAAAG4900.10690380403005524No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACCTTCATTG4720.10297672551466544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)