FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341841
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA187855.495244865302875No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA174675.109685497058574No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT167004.885312177298803No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG156594.580784633791734No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG151094.419891118970516No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC139054.067680588343704No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT106363.1113880429790455No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG104823.0663378588291046No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT96862.8334810628333056No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG91852.6869216975143417No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC82362.4093072510319122No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA77932.2797148381850043No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG66811.9544174045828324No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT65981.9301371105279939No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC65251.9087821531062688No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA60511.7701211966967099No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA57991.696402713542261No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT56381.6493047937491407No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG51061.4936768848675261No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT50121.4661787205162635No Hit
GGGGTCAGAGGCAAGCAGAGGTTGGGGCACAGCAGGCCAGTGTGCAGGGT46391.3570636641011464No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA40261.1777405284913163No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACTAGGACCA35411.0358617017853329No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT34991.0235752879262583No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG30250.8849143315166994No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAAGTGATAAAAG29990.8773084562706054No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG29200.8541982968690122No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC28880.8448372196430505No Hit
ACTTCTACCGTGCCCTGATGGACGAAGAAGACATGGACGACGTGGTGGAT28200.8249449305378816No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC25250.7386474998610465No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT21980.6429889919582497No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC21240.6213415008732129No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA20910.61168788998394No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG19110.559031830587905No Hit
GGGGTCAGAGGCAAGCAGCTGGACCTTTCTGAGCCTCAGTGTCCCCTTCT18880.5523035563317449No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCATCATGCGAT18270.5344590028697552No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA18240.5335814018798213No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT13960.4083769939825826No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT10660.3118408850898517No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG10530.30803794746680474No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC9460.27673684549249505No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAAATCCTGTTTGAAGGAAAA9430.2758592445025611No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT9400.2749816435126272No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC8790.2571370900506376No Hit
AGAAAACGGCATTTTGAGTGTGTACTGAGGCATAACTGCACCCTTAGACC8760.25625948906070367No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG8530.2495312148045436No Hit
AGCTGCTCACCATCGCTAGACCAAGTCTCTGCAGGTCTTGGCCAGTTGGA7660.22408078609646004No Hit
AGATGAGTCATATTTGTGGGTTTTCAACACTGACGACATGGTTCTACATG7130.20857650194096086No Hit
ATACGGCCAGGCATTGAAGTCCCATGGAAGCCAGCCCCTCAGGGCAACTG6580.19248715045883905No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG6270.18341860689618858No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGTCGTCATGTAGGGACGAACT5580.16323378412770848No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACAAAGTCTCTGCTACCGTATTC5040.147436966308898No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCATCATGCGATCTCG4950.14480416333909624No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4660.13632068710306838No Hit
AGCTGCTCACCATCGCTACCGTACAGGTCTTGGCCAGTTGGAGACCAAGT4400.12871481185697445No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT3680.10765238809856044No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT3670.10735985443524913No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG3630.10618971978200392No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC3570.10443451780213608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)