FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110271
Sequences flagged as poor quality0
Sequence length150
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT1465513.289985580977772No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA1203710.915834625604193No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT59535.398518196080565No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAAACAATAATG52984.80452702886525No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA47214.281270687669469No Hit
ATACGGCCAGGCATTGAAGTCTTATGGAAGCCAGCCCCTCAGGGCAACTG30702.784050203589339No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT25272.2916269916841236No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA20831.8889825974190855No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGGTATATTCCTCCAATTCAGGACCCA20461.855428897896999No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA19471.7656500802568218No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA18061.6377832793753573No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATATAGAGATCAT16961.5380290375529377No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG16121.461853071070363No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG16101.4600393575826827No Hit
ACTTCTACCGTGCCCTGATGGATGGAGAAGACATGGACGACGTGGTGGAT15131.3720742534301855No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG14881.349402834834181No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT10820.9812189968350699No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC9530.8642344768796874No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG9320.8451904852590437No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA7360.6674465634663692No Hit
GGAATCCTATGGCTTTCCAACCCAGCAACAGAGGAGGGGGAGACCAAGTC6000.5441140463041053No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGTTCATCTGGAC5990.5432071895602653No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT5900.5350454788657035No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC5290.4797272174914529No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATATAGAGATCATCTCG5220.47337922028457163No Hit
GGAATCCTATGGCTTTCCAACCCAGGAAGACAGGGGAGTAGGGCCAGGAA4630.4198746723980013No Hit
GGGGTCAGAGGCAAGCAGGAGGAGATAACACAGGCCCAAGAGACCAAGTC4450.40355125100887806No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATTCTTATTCTGA4280.3881346863635951No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTCTTGGCCAGTTGG4260.38632097287591477No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCAGAC4020.3645564110237506No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC3210.29110101477269634No Hit
GGTCTTCTCTGTTTCAGGGCATCACTGACGACATGGTTCTACAGCAACAG3090.2802187338466143No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT3080.27931187710277405No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA2900.2629884557136509No Hit
AGGACAAAATGTTTCACTTTTGGGTAAGTGCAGATAATGACAAGGAATAT2880.2611747422259706No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATAT2800.25391988827524914No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC2500.2267141859600439No Hit
CAGCCTCTGGCATTCTGAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCT2440.22127304549700283No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATATAGAGA2370.2149250482901216No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT2320.21039076457092074No Hit
TGTGGTCTGCCAGCTAAAGTGAAGATATATTCCTCCAATTCAGGACCCAC2210.2004153403886788No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG2190.19860162690099847No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC1870.16958221109811283No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTATATAGAGATCAT1830.16595478412275214No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATATAGAGATCATCT1800.1632342138912316No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCGGGACCCA1680.1523519329651495No Hit
AGGACAAAATGTTTCACTTTTGGTAAATACATTCTTCATACCAGGACCAG1560.1414696520390674No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGGCATGGACGACGTGGTGGAT1560.1414696520390674No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTATATAGA1550.14056279529522722No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG1510.13693536831986652No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC1500.13602851157602633No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATA1500.13602851157602633No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATAT1460.13240108460066563No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC1450.13149422785682546No Hit
GGGGTCAGAGGCAAGCAGGCAGGAGGAGATAACACAGGCCCAAGAGACCA1400.12695994413762457No Hit
TCCACAAACAGAACAAGATGCTGCTGCCGTAATCCTGTTTGAAGGAAAAG1370.12423937390610405No Hit
AGGACAAAATGTTTCACTTTGGGTAAATACATTCTTCATACCAGGACCAG1360.12333251716226389No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA1350.1224256604184237No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC1350.1224256604184237No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATA1340.12151880367458352No Hit
TGTTCATTCATGATCCCACTGCCAGCGAGATCTCCTCCATCCTGGAGAAA1320.11970509018690317No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG1310.118798233443063No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC1280.11607766321154248No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGCGGTGGAT1260.11426394972386211No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT1190.1079159525169809No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT1180.1070090957731407No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG1170.10610223902930054No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT1140.10338166879778No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC1140.10338166879778No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAGTT1130.10247481205393984No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC1120.10156795531009967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)