FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110271
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC103599.394129009440379No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA82077.442573296696321No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC58565.310553091928068No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT54624.953251534855039No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA49354.475338030851266No Hit
CTCGATAATCTGGATGACTCATTATTGTTTACGGTAGCACAACTAAAAGA48914.435436334122299No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC33573.0443180890714694No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGATCAAAATCAC24782.247191011235955No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT19841.7992037797789084No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA19491.7674637937445021No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT17701.6051364365971106No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG16861.528960470114536No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC16121.461853071070363No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT14641.327638272982017No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG14071.2759474385831269No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC13771.2487417362679216No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTTAGAACACCA10900.9884738507857914No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA9320.8451904852590437No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG9130.8279602071260803No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC8520.7726419457518295No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG6060.5495551867671464No Hit
CCCCCTCCTCTGTTGCTGGGTTGGAAAGCCATAGGATTCCTGTAGAACCA5910.5359523356095438No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT5620.5096534900381786No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC5500.4987712091120966No Hit
AAGACCCAGGTCCAGATGAACAGAGACTTGGTCTAAGACCCAGGGCAGCT5420.49151635516137515No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG5350.485168357954494No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC5310.4815409309791332Illumina Single End PCR Primer 1 (100% over 21bp)
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG5160.4679380798215306No Hit
AGGGATGCAAAGGCCTCAGAATAAGAATGTAGAACCATGTCGTCAGTGTA4680.42440895611720214No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT4580.4153403886788004No Hit
CTTGGGCCTGTGTTATCTCCTCCTGCTTGCCTCTGACCCCTGTAGAACCA4360.39538954031431656No Hit
CCAACTGGCCAAGACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCT4230.38360040264439427No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC4220.3826935459005541No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA3480.3155861468563811No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTTGTA3350.3037970091864588No Hit
CCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT3300.29926272546725796No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGA3220.2920078715165365No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC3080.27931187710277405No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC3020.27387073663973305No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA2740.2484787478122081No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA2700.2448513208368474No Hit
ACTAGATATTCCTTGTCATTATCTGCACTTACCCAAAAGTGAAACATTTT2480.22490047247236356No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA2460.22308675898468322No Hit
CCTGGTCCTCTGACTGCTCTCAGAATGCCAGAGGCTGTGTAGAACCATGT2380.2158319050339618No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG2330.21129762131476093No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT2310.20948390782708054No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT2150.19497419992563775No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1880.170489067841953No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC1800.1632342138912316No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC1690.15325878970898968No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC1630.14781764924594862No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC1550.14056279529522722No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT1540.13965593855138703No Hit
CTTGGGCCTGTGTTATCTCCCCTCCCTGTAGAACCATGTCGTCAGTGTAG1500.13602851157602633No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA1470.13330794134450583No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT1450.13149422785682546No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA1440.1305873711129853No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTTTTTTTTTTTTCTGTTT1380.12514623064994423No Hit
CTTGGGCCTGTGTTATCTCCTCCTGCCTGCTTGCCTCTGACCCCTGTAGA1370.12423937390610405No Hit
GGCCTTTTCCTTCAAACAGGATTACGGCAGCAGCATCTTGTTCTGTTTGT1370.12423937390610405No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG1360.12333251716226389No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG1320.11970509018690317No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT1300.11789137669922281No Hit
GTTCAAATGAGTAGACACAGCTTGAGGGAGAGAGAGAGACAGAGATTTCA1270.11517080646770231No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA1240.11245023623618178No Hit
CCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG1200.10882280926082107No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG1190.1079159525169809No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG1190.1079159525169809No Hit
GCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC1190.1079159525169809No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA1170.10610223902930054No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGACAGAGATTTCATACCAG1150.1042885255416202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)