FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239644
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT205398.570629767488441No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA190307.940945736175327No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA190047.930096309525797No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC111614.657325032130994No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC82593.4463621037872842No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA79583.320759126036955No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT68812.871342491362187No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG63722.6589441004156162No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC57522.4002270033883595No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC56952.3764417218874665No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT51672.1561149037739313No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA50572.1102134833336117No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA48612.028425497821769No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG44391.8523309575870874No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG42701.7818096843651414No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT40761.700856270134032No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT40211.6779055599138721No Hit
GTAGAGGAGCCGTCAAATCCAAGTCTCAAGAGGGATAAAACACCATGAAA40051.6712289896680075No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG39441.6457745656056484No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA30541.2743903456794246No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC24071.0044065363622705No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA21370.891739413463304No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC20410.8516799919881157No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG20110.8391614227771195No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA17060.7118893024653236No Hit
GGGGTCAGAGGCAAGCAGGCAAGCAAACGATGAACGGGAGATAACACAGG16770.6997880188946938No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT16450.6864348784029645No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG16270.6789237368763666No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCGATATCTAAT13340.5566590442489693No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGGCTAATGAGTTTGTA13020.5433059037572399No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATTTACTGTTTTCCTTTACT11800.49239705563252156No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC10860.4531722054380665No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC10420.4348116372619386No Hit
TCCACAAACAGAACAAGATGCTACCGGTAATCCTGTTTGAAGGAAAAGGC9920.41394735524361137No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG8880.3705496486454908No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGTTCTCTTTAACAATT8290.34592979586386474No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG7540.31463337283637394No Hit
CCCCTGCTCCTATAGCCAAGTCTCGCTACCGTAGATGCCGACGAGTACCT7540.31463337283637394No Hit
ATACGGCCAGGCATTGAAGTCACTGACGACAGGGGCCAGGAGGAGACCAA7360.3071222313097762No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG6030.25162324114102586No Hit
ATACGGCCAGGCATTGAAGTCGACGACATGGTTCGACAGGGGCCAGGAGG5590.23326267296489792No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC5350.22324781759610088No Hit
GGTCTTCTCTGTTTCAGGGCATGTGACAGGGAGGTGGCTTTAGGTCAGCA5200.21698853299060272No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA5140.21448481914840348No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA5100.2128156765869373No Hit
AGCTGCTCACCATCGCTACCGCAGGTCTTGGCCAGTTGGAGACCAAGTCT4800.20029710737594097No Hit
AGCTGCTCACCATCGCTACCGTACAGGTCTTGGCCAGTTGGAGACCAAGT4580.19111682328787702No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT4330.18068468227871343No Hit
GCGTGGACAACCCCCACATGTGCCGCCTGCTGGGCATCTGCCTCACCTCC4130.17233896947138255No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATCG3650.15230925873378845No Hit
ATACGGCCAGGCATTGAAGTTACTGACGACAGGGGCCAGGAGGAGACCAA3350.13979068952279214No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCCCACCTTCCACTCGGATAAGAT3240.13520054747876017No Hit
TGACCAATGGCTAAGTGAAGATGACAATCCTGTTGCAGCAATTCACTGTA3150.13144497671546126No Hit
ATACGGCCAGGCATTGAAGTGTAGCAGAGCAGTCAGAGGACCAGGAGACC2880.12017826442556458No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCGATATCTAATCTCG2620.10932883777603444No Hit
AGGACAAAATGTTTCACTTTGGGTAAATACATTCTTCATACCAGGACCAG2550.10640783829346866No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT2530.10557326701273555No Hit
AGGACAAAATGTTTCACTTTTGGTAAATACATTCTTCATACCAGGACCAG2430.10140041060907011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)