FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239644
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA180357.525746524010615No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC173707.248251573166864No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA139815.834070537964648No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA95713.99384086394819No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG74253.0983458797215873No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA71042.9643971891639262No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA56792.3697651516416016No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA54342.2675301697517987No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC53042.213283036504148No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC51082.131495050992305No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA48462.0221662132162708No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC42681.7809751130844085No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT42481.7726294002770777No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC42421.7701256864348784No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT41891.7480095474954513No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA37861.5798434344277346No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT37261.5548062960057418No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT36371.5176678740131195No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC36181.5097394468461551No Hit
CTTGGGCCTGTGTTATCTCCTGGGTTGGCTCTGACTGTACCACCATCCAC30621.277728630802357No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA29761.241842065730834No Hit
TTCATGGTGTTTTATCCCTCTTGAGACTTGGTCTTTCATGGTGTTTTATC28071.1713207925088882No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC22920.9564186877201182No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA20760.8662849894009447No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA20190.8424997079000518No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG18380.7669710069937073No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT16860.7035435896579927No Hit
CTTGGGCCTGTGTTATCTCCCGTTCATCGTTTGCTTGCCTGCTTGCCTCT16650.6947805912102952No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT14310.597135751364524No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG13300.5549899016875032No Hit
CCTGGTCCTCTGACTGCTCTTTCCACCCATCTACAGTCCCCCTTGCCGTC12980.5416367611957738No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGACCTAATTACG12320.5140959089315819No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC11940.4982390545976532No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC10540.4398190649463371No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT10450.43606349418303814No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG10010.4177029260069102No Hit
GGCCTTTTCCTTCAAACAGGATTACCGGTAGCATCTTGTTCTGTTTGTGG9580.3997596434711489No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG8760.3655422209610923No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT7650.3192235148804059No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA7460.3112950877134416No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT7210.300862946704278No Hit
CCTCCTGGCCCCTGTCGTCAGTGACTTCAATGCCTGGCCGTATTGTAGAA7150.29835923286207877No Hit
TTCATGGTGTTTTATCCCTCTTGAGACTTGGATTTGACGGCTCCTCTACT6990.291682662616214No Hit
ATGAGGTACTCGTCGGCATCTACGGTAGCGAGACTTGGCTATAGGAGCAG6950.29001352005474784No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG5820.24286024269332845No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG5700.2378528150089299No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC5670.2366009580878303No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCGACTTCAATGCCTGGCCGTAT5500.22950710220159903No Hit
GCTGACCTAAAGCCACCTCCCTGTCACATGCCCTGAAACAGAGAAGACCT5090.21239839094657076No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA5020.20947739146400493No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT5000.20864282018327185No Hit
CCAACTGGCCAAGACCTGCGGTAGCGATGGTGAGCAGCTTGTAGAACCAT4800.20029710737594097No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTTTTT4560.19028225200714394No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA4520.18861310944567775No Hit
CCAACTGGCCAAGACCTGTACGGTAGCGATGGTGAGCAGCTTGTAGAACC4510.18819582380531122No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG4320.18026739663834687No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCGGGTTCATT3920.16357597102368515No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT3710.15481297257598772No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA3670.15314383001452153No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG3570.14897097361085612No Hit
CCTCCTGGCCCCTGTCGTCAGTAACTTCAATGCCTGGCCGTATTGTAGAA3260.13603511875949326No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA3180.1326968336365609No Hit
AGCATCTTATCCGAGTGGAAGGTGGGGTACGGTAGCAGTAGGGGGGCTTT3010.12560297775032966No Hit
CCTGGTCCTCTGACTGCTCTGCTACACTTCAATGCCTGGCCGTATTGTAG2830.11809183622373187No Hit
CCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT2780.11600540802189915No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGGTTTACCTCTA2770.1155881223815326No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC2660.11099798033750063Illumina Single End PCR Primer 1 (100% over 21bp)
CGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTATC2570.10724240957420174No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA2500.10432141009163592No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT2470.1030695531705363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)