FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236276
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT158616.712912018148268No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA149146.312109566777837No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA136145.761905568064467No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG128635.444056950346205No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC112444.758841355025479No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC79863.3799454874807426No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT75603.1996478694408235No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGAGAGGCTG75493.194992297144018No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC60912.5779173508947166No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA55512.3493710745060863No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT55192.3358275914608337No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA54412.302815351538032No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTTCCAG52702.2304423640149658No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG52192.208857437911595No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT45571.9286766324129407No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT45341.9189422539741658No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT39961.6912424452758639No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC36731.5545379132878498No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG31541.3348795476476663No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC30731.3005976061893718No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA26081.1037938681880513No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAAGCTG18390.7783270412568352No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAACAGTCATATAT15070.637813404662344No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT14710.6225769862364353No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG14530.614958777023481No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG14220.601838527823393No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA13520.5722121586619039No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT13070.5531666356295181No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTAGGAGGCTG12580.5324281772164757No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA12370.5235402664680289No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTCCATACCAGGACCA12170.5150755895647463No Hit
GACCCAAAACCCAAAATGGCAGAGGAGGGAGAGATGGGGGTGGGAGGCTG12020.5087270818872843No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCCACCTGGAGGAGAAAACAGG11550.4888350911645702No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTTGTGGGGGTTGTTGACTT11410.48290981733227245No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC11300.478254245035467No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA11130.4710592696676768No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCTTCAGGGCAACTG8820.37329225143476275No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCGGCCCCTCAGGGCAACTG7460.31573244849244103No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC7230.3059980700536661No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC6820.2886454824019367No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC6380.27002319321471496No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC6280.2657908547630737No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCCGTGAAATAATACTGGTATGTA5580.2361644856015846No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG5540.23447155022092805No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG5400.22854627638863026No Hit
TGTTCATTCATGATCCCACTGCATCCCAAGTGTGAGTGACTGGTGGAGAC5390.2281230425434661No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA4640.19638050415615635No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAACAGTCATATATCTCG3890.1646379657688466No Hit
TTTCGCTTCCCACAGGTCTCTACGGAAGGCAGTCTGGCTGAGACCAAGTC3570.15109448272359444No Hit
AGCTGCTCACCATCGCTACTACCGTAAGGTCTTGGCCAGTTGGAGACCAA3570.15109448272359444No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC3300.13966716890416292No Hit
GGAAACCGTAGCTGCCCTGGTAGATTTTCTGGGAAGGGACAGAAGATGAC3070.12993279046538794No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG2920.12358428278792599No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGATCA2580.10919433205234556No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2560.1083478643620173No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA2500.10580846129103252No Hit
AGCTGCTCACCATCGCTGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAA2480.10496199360070427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)