FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191464
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA1977910.330401537625871No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC173629.06802323152133No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA171128.937450382317301No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG97965.11636652321063No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC92284.819705009819078No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC78484.098942882212844No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT66763.4868173651443617No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG51812.7059917269042746No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG35041.8301090544436551No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAAGACCGTCGCTTGGTG34251.7888480340951824No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA33361.7423640997785483No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT25321.3224418167383947No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC24911.3010278694689341No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA23841.24514269000961No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAAGTCAGACGCAT23561.230518530898759No Hit
TCATAATGCTTGCTCTGATAGGAAAATGGGATCTACTGTTTTCCTTTACT23041.2033593782643213No Hit
ACTTCTACCGTGCCCTGCCTTGATTTCTTTTATTGTCTTTATAAAGCTGT21251.1098692182342373No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC20561.0738311118539254No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT18530.9678059583002548No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA18000.940124514269001No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC15800.8252204069694563No Hit
AGAAAACGGCATTTTGAGTGTACTGAGGCATAACTGCACCCTTAGACCAA13750.7181506706221535No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA13420.7009150545272218No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG11760.6142146826557473No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT11620.6069026031003217No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT10450.5457945096728366No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACTACGGTAGGGACGAACTGGTG10350.5405715957046755No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAAGTCAGACGCATCTCG8100.4230560314210504No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT8000.41783311745288937No Hit
TCAAATCATCCATTGCTTGGTAGGTAGCAGAAAGTCAGTCCCATGGAGAC6190.3232983746291731No Hit
GGAATCCTATGGCTTTCCAACCCACTGACGACATGGTTCTACAGCAACAG5010.26166798980487194No Hit
CAGTCAGAGGCGCTATGTGTACACTGACGACATGGAACAGTTTTCTTAAC5000.2611456984080558No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT4900.2559227844398947No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC4710.24599924790038857No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAA4380.2287636318054569No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA4210.219884678059583No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAAGT3640.19011406844106463No Hit
TGTGGTCTGCCAGCTAAAGGTTTGTACTTTACTTTCATTGGGAGAAGACC3550.1854134458697196No Hit
GGTCTTCTCTGTTTCAGGGCATCTTCTTACCCAATCCCATGAGTGGGGAG3160.1650440813938913No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA2870.14989763088622404No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAAG2840.14833075669577572No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAAGTCAGAC2690.14049638574353404No Hit
AGGACAAAATGTTTCACTTTTGGTAAATACATTCTTCATACCAGGACCAG2640.13788492875945346No Hit
GGAAACCGTAGCTGCCCTGGTCTTCATCTGGACCTGGGTCTTAGACCAAG2600.13579576317218903No Hit
GGAATCCTATGGCTTTCCAACCACACTGACGACATGGTTCTACAGCAACA2590.1352734717753729No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTAAGTCAG2550.13318430618810848No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAAGTCAGACGCATCT2530.13213972339447624No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC2430.12691680942631514No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC2420.12639451802949903No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2380.12430535244223456No Hit
AGGACAAAATGTTTCACTTTGGGTAAATACATTCTTCATACCAGGACCAG2380.12430535244223456No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT2310.12064931266452178No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAAG2170.11333723310909623No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTAAGT2100.10968119333138344No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATACCCTCCATGA2000.10445827936322234No Hit
TGACAGTTTGACAGTTAAAGGCATGTAGAACCAGCAGCCGTTACCTGTGT1990.10393598796640621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)