FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191464
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC172849.027284502569675No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA162098.465821250992352No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA150377.853695733923871No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC98175.127334642543769No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG79324.142815359545398No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC74543.8931600718672965No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTAATTCCACACCCC62103.243429574228053No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG48562.536247022939038No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC38271.998809175615259No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC34271.7898926168888145No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT31561.6483516483516485No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT27901.4571929971169515No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA24301.2691680942631514No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG23781.2420089416287134No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT23721.2388751932478168No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA20261.0581623699494422No Hit
GTTCAAATGAGTAGACACAGCTTTATAAAGACAATAAAAGAAATCAAGGC19821.0351815484895333No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA18280.9547486733798521No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT18210.9510926336021392No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC18050.9427359712530815No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA16920.883717043412861No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA15480.8085070822713407No Hit
AAGGGTGCAGTTATGCCTCAGTACACTCAAAATGCCGTTTTCTTGTAGAA13430.701437345924038No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT13050.6815902728450257No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG11520.6016796891321606No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC11230.5865332386244934No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC10220.5337818075460662No Hit
CTGATGGGACCCACTCCATCGAGATTTCACCGTAGCTAGACCAAAATCAC9490.49565453557848993No Hit
TCATTACACCAGTTCGTCCCTACCGTAGTGGTAAAAAGATAACCTCAGAA8890.46431705176952326No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC8240.430368110976476Illumina Single End PCR Primer 1 (100% over 21bp)
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG8210.42880123678602766No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG8030.41939999164333763No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT6570.34314544770818534No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG6230.32538754021643757No Hit
CCATGGGACTGACTTTCTGCTACCTACCAAGCAATGGATGATTTGATGTA6110.31912004345464423No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA5030.26271257259850417No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA4900.2559227844398947No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT4830.2522667446621819No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC4700.24547695650357246No Hit
TCTAGATATGGTTAAGAAAACTGTTCCATGTCGTCAGTGTACACATAGCG4420.23085279739272135No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC3710.1937701082187774No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTAGCTGGCAGACCACATGTAG3550.1854134458697196No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA3490.18227969748882297No Hit
GCTGACCTAAAGCCACCTCCCCACTCATGGGATTGGGTAAGAAGATGCCC3010.1572097104416496No Hit
CTTGGGCCTGTGTTATCTCCCCTCCCTGTAGAACCATGTCGTCAGTGTAG2740.1431078427276146No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTAGTTCCACACCCC2680.13997409434671793No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG2660.1389295115530857No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2620.13684034596582126No Hit
AAGACCCAGGTCCAGATGAAGACCAGGGCAGCTACGGTTTCCTGTAGAAC2460.12848368361676346No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT2460.12848368361676346No Hit
CGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTATC2400.1253499352358668No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC2380.12430535244223456No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA2350.12273847825178623No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG2130.11124806752183178No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC2130.11124806752183178No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA2050.10706973634730288No Hit
GCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC2040.10654744495048679No Hit
CACACACAGGTAACGGCTGCTGGTTCTACATGCCTTTAACTGTCAAACTG2000.10445827936322234No Hit
AGGGATGCAAAGGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTGTA1960.10236911377595789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)