FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303322
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA238247.854359393647675No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT231077.617976935401982No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC189786.256717283942477No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA184296.075721510474017No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG166195.478995918528824No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC98133.235175819755903No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG89042.935494293193372No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG82842.731091051753582No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC77172.5441609906304192No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT75072.474927634658877No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT73342.417892536644226No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA68842.269535345276637No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT61862.039416857333131No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT60341.9893050949156343No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG59201.9517212731025115No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA54031.7812753443535254No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT48661.6042357626548684No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC39711.309169793157107No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA37691.2425738983654333No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC31861.0503689148825341No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA26570.8759667943637455No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA26450.8720106025939431No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG21110.6959600688377368No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA21030.6933226076578685No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGACCAGGAA19980.6587059296720976No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATCATGACA19690.6491451328950751No Hit
GCAGCTTCTGCCATCTCTCTTCTCCTTTTTCTTCAGCCACAGGCTCCCAG19210.6333203658158657No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC16860.5558449436572356No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT16510.5443060509953119No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAACGATACACTAT15770.5199095350815306No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG14610.4816663479734407No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC14120.4655118982467477No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC11290.37221170900890804No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT10540.34748551044764314No Hit
GGAATCCTATGGCTTTCCAACCCCGACATGGTTCTACAGCAACAGAGGAG9840.32440772512379584No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG9370.3089126406920698No Hit
GAGAAAGCCCCCCTACTGCTACCGTACACTGTACACCCCTTCCACTCGGA7360.24264642854787982No Hit
GGGGTCAGAGGCAAGCAGGCCCAAGAGACGGAGATAACACAGGCCCAAGA7240.23869023677807744No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA6850.22583261352621967No Hit
GGGGTCAGAGGCAAGCAGACCAAGTGGAGATAACACAGGCCCAAGAGACC6000.1978095884901194No Hit
TCCAAATACTCCACACGCAAATTTCTTCCACTCGGATAAGATGCTGAGGA5260.173413072576338No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG4620.15231338313739196No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCCCTCTGTCATAGGGACTCTG4490.14802750872010603No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC4430.14604941283520484No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT4400.14506036489275423No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG4020.13253242428838No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC3720.12264194486387404No Hit
GAGAAAGCCCCCCTACTGCTGGTTCTACAGAGAAAGCCCCCCTTCCACTC3600.11868575309407164No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAACG3510.11571860926671985No Hit
AGGACAAAATGTTTCACTTTTGGTAAATACATTCTTCATACCAGGACCAG3490.1150592439717528No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT3450.11374051338181865No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAACGATACACTATCTCG3360.11077336955446689No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAA3140.10352035130982917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)