FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522698
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC538933.53930983031435No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG372552.446644048918433No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC369722.4280586170074434No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA352162.3127369970933174No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA343712.2572433929774647No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG324952.134041024549845No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA309072.0297524525546105No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG307122.016946236220183No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT305032.003220599225848No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT303271.9916621680727233No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG296441.946807574450088No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC259221.7023730247232214No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA250061.6422166444035522No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG246221.6169982491603718No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA238161.5640658883114051No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA221171.4524876239411886No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT215131.412821189756603No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC209641.376766765307369No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT207731.3642232405900578No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT196571.2909322794145655No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC196461.2902098774674953No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG191561.2580301543707288No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC188171.2357670398201088No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT178231.1704881729666683No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT174361.1450727590106509No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT171821.1283918413237555No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT159761.0491903187631428No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC159241.0457753277406288No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT158601.0415722618667653No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT158421.0403901495897414No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT157991.0375662147057394No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC155181.019112128603308No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT150190.9863413493680296No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT148000.9719589833309034No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA146840.9643409264345262No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA124940.820517266063264No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC121620.7987138618425978No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT115980.7616743438291769No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA104310.6850340645354496No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC101440.6661859410073435No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT94970.623695571938756No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA94640.6215283660975452No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT91540.6011697657710197No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTGATCTACTGAGACG86920.5708288839940684No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT81330.534117730502043No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT79700.5234130471045474No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG69120.45393111437724354No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC68270.4483489175135188No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC55430.364024908419135No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT52660.3458335139338201No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA51990.3414334293471194No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG51730.33972593383586236No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA44960.2952653776389015No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC41090.26984996368288394Illumina Single End PCR Primer 1 (100% over 21bp)
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT39810.2614438319351572No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT32390.21271453696005382No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA30150.19800380640153203No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTTTTT27060.17771087897928545No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA24770.1626717838993681No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT22600.14842076367080012No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA22520.1478953804365672No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC22390.1470416326809387No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC22360.14684461396810136No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA22120.1452684642654026No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA21260.1396205944973987No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA20500.13462945377218594No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG19680.1292442756212985No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC18810.1235307329490155No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG18730.12300534971478258No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTGATCTACTGGGACG18310.12024708773505975No Hit
GTATCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT18250.11985305030938506No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG17950.1178828631810116No Hit
TTCTCTTCCGCACCCAGTAGAACCATGTCGTCAGTGTGGGAAGAAGCTCT16610.10908269400761018No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT16210.10645577783644557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)