FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125329
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA480274.267818566836898No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG456304.054814192116261No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT349073.1019373001140114No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTCTTAGTTCAT321292.8550761599496677No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA257102.2846651956894384No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA228712.0323834185380454No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA222021.9729341374833491No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC218861.944853460632402No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG197181.752198690338559No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC193361.7182530619934258No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGTAACAATAATGA183671.6321449105106152No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC162951.4480209787537688No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC162461.4436666965838434No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA153541.3644009885109154No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA149001.3240572312630352No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC147711.3125939169789456No Hit
TCTTAAATGGCTACGACCCAGTACACTGACGACATGGTTCTACATCTTAA144021.2798035063523645No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAGTAGAACCAAGCTGTGTC140911.2521671440085522No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT137401.220976265607658No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA134231.1928067258552832No Hit
TGACCAATGGCTAAGTGAAGATGAGAGACTTGTTTTTCCTTTCCTCTTCC132571.178055484218393No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA117481.0439613659649756No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC116621.0363191564422494No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC111760.9931317863487034No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG104780.9311054811526229No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG102680.9124442718529425No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG94740.8418871281198654No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT93480.8306904025400571No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG92700.8237590962287473No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG91870.8163834754103023No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA87150.7744401859367349No Hit
TGTGGTCTGCCAGCTAAAGGCACTGACGACATGGTTTGTACTTTACTTTC82940.7370289044359473No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG81240.7219222111933488No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT80420.7146354532763307No Hit
CAGTCAGAGGCGCTATGTGTACACTGACGATATGGAACAGTTTTCTTAAC78590.6983735423151808No Hit
TCACAATTGCCAGTTAACGTCTTCCTTTTCTCTCTGTCATAGGGACTCTG70040.6223957615950535No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA69430.6169751246080035No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT67650.6011575281539888No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT67520.6000023104354371No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG67460.5994691330268748No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGAAGCTGTGTCTACTCATTTGAA64410.5723659480916248No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG60450.5371762391265131No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC59700.5305115215194846No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT59680.5303337957166304No Hit
CAGAGGGGAAAAATATGACAAAACACTGACGACATGGTGCTAAAATGGAG59670.5302449328152035No Hit
GCAGCTTCTGCCATCTCTCTAGAACGTGGGAGTAGACGGAAGACCATCTC58990.524202255518164No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACTCTTAGTTCATCTCG56740.5042081026970779No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT46540.4135679432414876No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAAGTGCTAAAATGG45140.4011271370417007No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG43760.38886405664476786No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC42450.3772230165578244No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG40610.3608722426952473No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT40300.3581174927510088No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGATTCTG39680.35260799286253175No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACTC36430.32372754989874075No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG36170.32141711446163745No Hit
CAGTCAGAGGCGCTATGTGTGGAACAGTTTTCTTAACCATATCTAGAAGA35930.31928440482738824No Hit
TCAAATCATCCATTGCTTGGTACGTAGCAGAAAGTCAGTCCCATGGAGAC35930.31928440482738824No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA35720.3174182838974202No Hit
ACTTCTACCGTGCCCTGATGGATGAAGGAGACATGGACGACGTGGTGGAT35190.3127085501217866No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA33100.2941362037235333No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC31820.282761752340871No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG29470.2618789705055144No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTACTC29410.2613457930969521No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA28030.24908271270001925No Hit
TGACCAATGGCTAAGTGAAGATGACCAAGTCAGTGTGTTTTTCCTTTCCT27130.24108505157158486No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT24200.21504822145345942No Hit
CTGGTGTTGTTGGGCAGTGTATCTCCCCTTTCTTGCGGAGATAGACCAAG23220.20633965711360855No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACTCTTAGTTCATCT22430.19931948790087167No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC21930.19487634282951918No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACTCTTAGTTCAT21120.1876784478139282No Hit
TCCACAAACAGAACAAGATGCTACCGTAAATCCTGTTTGAAGGAAAAGGC19380.17221630296562163No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTACTCTTAG19090.16963927882423718No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTGCTAATGAGTTTGTACTGAG19060.16937269011995604No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCAAGGTGGGGGCAGCGCCT17810.15826482744157486No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG17110.15204442434168142No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCTTCAGGGCAACTG16400.1457351583403609No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTACTC15950.1417363277761437No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT15750.1399590697476027No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGG15260.1356047875776773No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTACTCTTAGTTCAT14560.12938438447778383No Hit
TCCCTGCCAGCGAGATGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTG14440.12831802966065925No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT14380.12778485225209696No Hit
GCAGCTTCTGCCATCTCTCTAGAACGTGGGAGTAGACGGAAGACCAAGTC14330.1273405377449617No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC14180.12600759422355595No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACTCT13650.12129786044792233No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGGGGGTTGTTGACTTGTA12650.11241157030521742No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACTC12400.11018999776954118No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA12220.10859046554385428No Hit
GGTCTTCTCTGTTTCAGGGCATGAACCACTCTTGGTTTCCTGGAAGACTA11930.10601344140246985No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA11700.10396959466964772No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG11600.10308096565537722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)