FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125329
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG462764.112219626438135No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC446423.967017645506336No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG324452.8831568368006155No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG300242.6680197524457294No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA296592.635584793424856No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA260862.318077646626009No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA215331.9134848564286533No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA173641.5430154203792847No Hit
CTCGATAATCTGGATGACTCATTATTGTTACGGTAGCACAACTAAAAGAA168621.498406243862906No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC158811.4112317375629706No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA155991.3861723993605424No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT154281.370976843216517No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT144571.2846909659308523No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA144181.2812253127751974No Hit
GTTCAAATGAGTAGACACAGCTTGGTTCTACTGGTCTTCTCTGTTTCAGG137871.2251528219747292No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC135871.2073802416893193No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC132681.1790329761340905No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG127971.1371785495619502No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA126821.1269593158978397No Hit
TCCAGGAAGAGGAAAGGAAAAACAAGTCTCTCATCTTCACTTAGCCATTG116051.0312539710609077No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT114971.0216567777067862No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG114001.0130370762683625No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG111250.9885997783759238No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA110370.9807798430503435No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC108980.9684278997519837No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT106380.9453235453809508No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT97820.8692569017593965No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT92130.8186939108474055No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG90270.8021654111819744No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAAATACAGTCTA89940.7992329354348817No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG82580.7338298399845734No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT81990.7285869288003775No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA81180.7213890337847865No Hit
TCTCCCAATGAAAGTAAAGTACAAACCATGTCGTCAGTGCCTTTAGCTGG74690.6637170107586314No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA72470.6439894466418264No Hit
TCTAGATATGGTTAAGAAAACTGTTCCATATCGTCAGTGTACACATAGCG70070.6226623502993347No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT66920.5946705363498141No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC65470.581785415642892No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC65240.5797415689100699No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT64540.5735211658101763No Hit
GTTCAAATGAGTAGACACAGCTTCTTCTCTGTTTCAGGGCACGGTAGAAG62570.5560151742290477No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG61110.5430411906206984No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG60470.5373539649293674No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC58700.5216252313767796No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC57770.5133609815440641Illumina Single End PCR Primer 1 (100% over 21bp)
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA57730.5130055299383558No Hit
AACAGAGAATCTCCATTTTAGCACCATGTCGTCAGTGTTTTGTCATATTT56800.5047412801056402No Hit
TCCGTCTACTCCCACGTTCTAGAGAGATGGTCTTCCGTCTACTCCCACGT51670.45915461167356386No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC47320.4204992495527975No Hit
GGCCTCAGTACAAACTCATTAGCTAGCAGAGACTTGGTCTGGACCTAATT44640.39668399197034826No Hit
AACAGAGAATCTCCATTTTAGCACTTACGGTAGCAGAGACTTTGTCATAT43220.3840654599677072No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT36530.3246161789130112No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA36080.32061734834879396No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC36040.3202618967430858No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA35740.31759600970027435No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT35180.3126196872203596No Hit
TCTAGATATGGTTAAGAAAACTGTTCCACACATAGCGCCTCTGACTGTGT35080.31173105820608904No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT34920.3103092517832563No Hit
CCATGGGACTGACTTTCTGCTACGTACCAAGCAATGGATGATTTGATGTA34880.30995380017754803No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG34790.30915403406470465No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCAGT31610.28089563141090296No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC29900.2657000752668775No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA28980.25752468833558895No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA28410.25245950295424713No Hit
TCCAGGAAGAGGAAAGGAAAAACACACTGACTTGGTCATCTTCACTTAGC23510.20891668125499302No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA23260.20669510871931676No Hit
ATCTCCGCAAGAAAGGGGAGATACACTGCCCAACAACACCAGTGTAGAAC22830.20287400395795366No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC22830.20287400395795366No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG21430.19043319775816672No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTCCTATCAGAGCAAGCATTAT21130.18776731071535524No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC18980.16866178690853964No Hit
GGCCTCAGTACAAACTCATTAGCACTTGGTCTGGACCTAATTACGAGTGA18670.1659070369643011No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAGGATTTACCTCTA18300.16261910961150028No Hit
GGCCTTTTCCTTCAAACAGGATTTACGGTAGCATCTTGTTCTGTTTGTGG18180.1615527547943757No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA15780.14022565845188384No Hit
AGGGATGCAAAGGCCTCATCATCTCGCTGGCAGGGATGTAGAACCATGTC15330.13622682788766663No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA15060.1338275295491363No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG14610.12982869898491908No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT14550.12929552157635676No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA14160.12582986842070187No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT14110.12538555391356662No Hit
TCCGTCTACTCCCACGTTCTAGAGAGATGGCAGAAGCTGCTGTAGAACCA14040.12476351360357726No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA14010.1244969248992961No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC13800.12263080396932807No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT13630.12112013464506824No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT13340.11854311050368382No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACTTACCAGGGC12410.11027886067096822No Hit
ACAAGTCAACAACCCCCACACTGACTTCCATTTTCTACTTTTTCTGAGGT12170.10814615103671905No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA11990.10654661881103215No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT11910.10583571559961576No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGGGTTGGAAAGCCA11390.10121484472540919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)