FastQCFastQC Report
Wed 8 May 2024
EGAF00008098641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181398
Total Bases177.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATTCTGTTGCCAT480254.065099145249949No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG432123.657700453191896No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT388133.285344989580141No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC367723.1125835662494774No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA358473.0342864978610087No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG213551.807604211281888No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA202191.7114469467529148No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA197831.674541517761161No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA159081.3465402853229818No Hit
AGATGAGTCATATTTGTGGGTTTTCACATGGTTCTACATGACCACTGACT151751.284495148967579No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA150461.2735758821328629No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC143961.2185563205625878No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA143491.21457798303366No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG142621.2072138263311771No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC135411.146184435727841No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG133801.1325565135542806No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA125821.0650094210418504No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC125101.0589149465294507No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC119571.0121059964550472No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC113370.9596257992649386No Hit
TGACCAATGGCTAAGTGAAGATGTTTTTCCTTTCCTCTTCCTGGAAGACC112320.9507380239343558No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCAAGCTGTGTCTACTCATTT112120.949045114347578No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA106850.9044369467359857No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG103230.8737952832153093No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG100170.8478937665376105No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTACCGAACAATAATGAG98000.8295256975210725No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC95140.8053170904301514No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG91220.7721360625293084No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT84040.7113606083639891No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATTCTGTTGCCATCTCG78670.6659059859590079No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT77960.659896156925947No Hit
AGAAAACGGCATTTTGAGTGTACACTGAGGCATAACTGCACCCTTAGACC75940.6427977700994923No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCAGATTATCGAGAGACCA75560.6395812418846147No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG72650.6149494073969992No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG69890.591587255099467No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG69710.590063636471367No Hit
GACCCAAAACCCAAAATGGCACGGTAGGAGACAGAAGCAGGGAAGACCAA68030.5758431959424344No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG64540.5463019236531635No Hit
GTAGAGGAGCCGTCAAATCCAAGAGGGATAAAACACCATGAAAGACCAAG60020.5080421669919875No Hit
AGGACAAAATGTTTCACTTTTGGGTAATACGGTGCAGATAATGACAAGGA58440.49466818125644363No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT57320.4851878875704885No Hit
TGTGCTGTGACTGCTTGTAGAACAGAGTTGAAAGTCAGGGCAAGACCAAG50540.4277982525787245No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC49450.41857189533078604No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT48470.4102766383555753No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT48010.4063829463059866No Hit
ACTTCTACCGTGCCCTGAAGCTGTGTCTACTCATTTGAACAGACCAAGTC47470.4018120904216868No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTATTC46850.39656407070267596No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT43830.3710011359423327No Hit
AGATGAGTCATATTTGTGGGTTTTCATTCTACAAGATGAGTCAGTGTACC43760.37040861758696053No Hit
GTAGAGGAGCCGTCAAATCAAGAGGGATAAAACACCATGAAAGACCAAGT40270.3408673452976897No Hit
TCACAATTGCCAGTTAACGTTTCTGCTTTGCTGTGTGGAGACCAAGTCTC40030.3388358537935564No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCCTTTGA39400.33350318859520667No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC39330.3329106702398345No Hit
GTAGAGGAGCCGTCAAATCCAAGTCTCAAGAGGGATAAAACACCATGAAA38900.32927091462826247No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATTC38380.3248693497026404No Hit
GTAGAGGAGCCGTCAAATCCAGAGGGATAAAACACCATGAAAGACCAAGT37300.31572763793404085No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC37100.31403472834726315No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCCGTATGTGGGGGTT35490.3004068061737027No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT35340.2991371239836194No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACTGCTACCGTAAGGGACGAACT34220.2896568302976643No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA31660.2679875875869097No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT31190.26400925005798215No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTGTGCTTGCAAATATCTTCTAAA30970.2621470495125267No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT30640.25935374869434347No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT27660.23412939585135575No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC27470.23252113174391692No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGGCTAATGAGTTTGTA25520.21601526327283438No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATTCTGTTGCCAT24780.2097514978017569No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG24370.2062810331488626No Hit
GGGGTCAGAGGCAAGCAGGGAAGAGGAGATAACACAGGCCCAAGAGACCA24360.20619638766952372No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAACCAAGCTGTGTCTACTCATTTG23040.19502318439679092No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT21230.17970235263645276No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG20980.17758621565298063No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC20790.1759779515455418No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACGTCAGTGAGGGACGAACTGGT20760.17572401510752514No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTGTGCTAAAATGGAG20470.17326929620669748No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGAACCA20270.17157638661991978No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGCTACCGATGCCGACGAGTACCTCA19290.16328112964470906No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATTCTGTTGCCAT18850.15955672855379813No Hit
TGTGCTGTGACTGCTTGTAGAACCAACAGAGTTGAAAGTCAGGGCAAGAC18790.15904885567776483No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCAGTGTGTGGGGGTTGTTGACTTG18580.15727130061164823No Hit
AGGACAAAATGTTTCACTTTTGGGTACGGGTGCAGATAATGACAAGGAAT18470.1563402003389205No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTATTCTGTTGCCATCTCG17770.15041501678519856No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATTCTGTTGCCATCT17500.14812958884304867No Hit
CAGAGGGGAAAAATATGACAAAACACTGACGACATGGTGCTAAAATGGAG17260.1460980973389154No Hit
CAGAGGGGAAAAATATGACAAAGTCGTGCTAAAATGGAGATTCTCTGTTA16850.14262763268602113No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC16730.14161188693395452No Hit
CAGAGGGGAAAAATATGACAAAGTCTGTGCTAAAATGGAGATTCTCTGTT16700.14135795049593786No Hit
AGAAAACGGCATTTTGAGTGAGGCATAACTGCACCCTTAGACCAAGTCTC16550.14008826830585458No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATCCCTGCCAGCG16420.13898787707444907No Hit
CCCCTGCTCCTATAGCCAACTTCTAGACGCGATGCCGACGAGTACCTCAT16390.13873394063643243No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT15870.13433237571081041No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG15190.12857648311576625No Hit
GACCCAAAACCCAAAATGGCACACTGACGACATGCTTCTACGGTAGGAGA14940.12646034613229412No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGGTGCTAAAATGGAGATTCTCT14780.12510601846287195No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACCGGTAGCAGGGACGAACTGGT14720.12459814558683864No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATTCT14640.12392098175212757No Hit
AGGACAAAATGTTTCACTTTTGGGTAATTACGGTGCAGATAATGACAAGG14480.1225666540827054No Hit
TCAAATCATCCATTGCTTGGCGGTAGCAGAAAGTCAGTCCCATGGAGACC14460.12239736312402763No Hit
AGCTGCTCACCATCGCTACCGCAGGTCTTGGCCAGTTGGAGACCAAGTCT14330.12129697189262212No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT14110.11943477134716667No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTACCGTATTCTGTT14100.11935012586782778No Hit
TCTTAAATGGCTACGACCCACACTGACGACATGGTTCTACATCTTAAATG13810.11689540696700011No Hit
TCACAATTGCCAGTTAACGTCTCTGCTTTGCTGTGTGGAGACCAAGTCTC13720.11613359765295014No Hit
TCCCTGCCAGCGAGATATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGC13570.11486391546286687No Hit
CAGAGGGGAAAAATATGACAAACTTGGTGCTAAAATGGAGATTCTCTGTT13550.11469462450418912No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG13240.11207061464468368No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATTC13150.11130880533063371No Hit
GCAACAGATAACTCAGATTGCCTTACACTGACGACATGGTTCTACAGCAA13110.11097022341327817No Hit
TCCCTGCCAGCGAGATGAGGCCTTTGCATCCCTAGACCAAGTCTCTGCTA13070.11063164149592263No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCGTATGTGGCTACCTGTTA12670.10724582232236723No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTCTCTGCTAGCTAATGAGTTT12300.10411393958682849No Hit
TACCCTCCATGAGGCATAACTGCACCCTTAGACCAAGTCTCTGCTACCGT12130.10267496643806744No Hit
TCATAATGCTTGCTCTGATAGGACACTGACGACATGGAGTGGGTCCCATC11850.10030489301657868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)