FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181398
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG484174.098280173150792No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG437693.7048479851836555No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC342972.903086004885737No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA334222.8290212104642127No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA245072.0744067621580533No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA217461.840700593703392No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA167951.4216208254965728No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG156351.323432069463466No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT155431.3156446853642887No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT150231.2716290361080687No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT144281.221264975901432No Hit
TCTGGATCAGAGTCAGTGGTCATGTAGAACCATGTGAAAACCCACAAATA140321.1877453660832336No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG130121.101406977157571No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA128061.0839700084137607No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT123121.0421551416203516No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA122241.0347063394385296No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC121991.0325902024550575No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG121801.0309819383476186No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA117860.997631619488098No Hit
TCCAGGAAGAGGAAAGGAAAAACATCTTCACTTAGCCATTGGTCATGTAG109880.9300845269756677No Hit
GTTCAAATGAGTAGACACAGCTTGGTCTTCTCTGTTTCAGGGCACGGTAG109790.9293227176616178No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC104560.885053131967381No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA104100.8811594399177922No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC101180.8564429599508379No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT90850.7690041797937698No Hit
CTCGATAATCTGGATGACTCATTATTGTTCGGTAGCACAACTAAAAGAAA89000.753344766116076No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG86680.7337070149094548No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC79470.6726776243061187Illumina Single End PCR Primer 1 (100% over 21bp)
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT77220.6536323914548695No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG76400.646691462149081No Hit
AAGGGTGCAGTTATGCCTCAGTGTACACTCAAAATGCCGTTTTCTTGTAG74170.6278155202565097No Hit
CTCGATAATCTGCACAACTAAAAGAAAAGTTTAAAAGTGATATATGTAGA72960.6175734172565046No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT72920.617234835339149No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC71220.6028451038515386No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG68580.580498697306073No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA67610.5722880858102011No Hit
TCCCTGCTTCTGTCTCCTACCGTGCCATTTTGGGTTTTGGGTCTGTAGAA66220.5605223641820961No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT64890.5492645154300244No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT62900.5324200650415863No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT62580.529711409702742No Hit
TTCATGGTGTTTTATCCCTCTTGGATTTGACGGCTCCTCTACTGTAGAAC57850.4896740979754495No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTATTACCCAAAAGTGAAACA53420.4521761506283234No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTATTGGCTTTCG51090.43245375394236324No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT50550.4278828980580634No Hit
TGCCCTGACTTTCAACTCTGTTCTACAAGCAGTCACAGCACATGTAGAAC49630.42009551395888595No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG49030.4150167851985529No Hit
GTTCAAATGAGTAGACACAGCTTCAGGGCACGGTAGAAGTTGTAGAACCA46650.39487116111589826No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA45780.3875070044134153No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA41940.3550031403472835No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA41820.35398739459521683No Hit
TCTGGATCAGAGTCAGTGGTACACTGACTCATCTTGTAGAATGAAAACCC40880.3460307195373617No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG39920.3379047535208287No Hit
CCACACAGCAAAGCAGAAACGTTAACTGGCAATTGTGATGTAGAACCATG39350.3330799611985123No Hit
TTCATGGTGTTTTATCCCTCTTGATTTGACGGCTCCTCTACTGTAGAACC38900.32927091462826247No Hit
TTCATGGTGTTTTATCCCTCTTGAGACTTGGATTTGACGGCTCCTCTACT37460.317081965603463No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA37190.3147965376613131No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGATAGAGATTTCA37160.31454260122329647No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG36390.30802489931420235No Hit
TTCATGGTGTTTTATCCCTCTGGATTTGACGGCTCCTCTACTGTAGAACC35990.30463908014064695No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG33660.28491668345468674No Hit
TCATTACACCAGTTCGTCCCTTACGGTAGCAGTGGTAAAAAGATAACCTC30690.2597769760910379No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT30640.25935374869434347No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA30380.2571529662315325No Hit
ACAAGTCAACAACCCCCACATACGGGTAGCAGAGACTTCCATTTTCTACT30340.2568143843141769No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCACACTATCAAGAAAAACCAAA29710.2514817191158272No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT26720.2261727207935006No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA25420.21516880847944556No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG25360.2146609356034122No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC24560.20788929725630143No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT23500.19891687644637965No Hit
CTTGGGCCTGTGTTATCTCCTCTTCCCTGCTTGCCTCTGACCCCTGTAGA23160.19603893014885754No Hit
GTTCAAATGAGTAGACACAGCTTGGTTTCTCTGTTTCAGGGCACGGTAGA22660.19180665618191328No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG22510.19053697399183No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC22290.18867477344637454No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC22080.18689721838025797No Hit
CCATGGGACTGACTTTCTGCTACCAAGCAATGGATGATTTGATGTAGAAC20520.1736925236033919No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT20290.17174567757859757No Hit
AACAGAGAATCTCCATTTTAGCACACGGTAGCAGAGACTTTGTCATATTT19870.16819056744636438No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC19530.16531262114884232No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC19080.1615035745785925No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG18860.159641374033137No Hit
TCATTACACCAGTTCGTCCCTCACTGACGTGGTAAAAAGATAACCTCAGA18850.15955672855379813No Hit
TGCCCTGACTTTCAACTCTGTTGGTTCTACAAGCAGTCACAGCACATGTA18310.15498587266949834No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC18090.15312367212404288Illumina Single End PCR Primer 1 (95% over 22bp)
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC17640.14931462555379305No Hit
ATGAGGTACTCGTCGGCATCGGTAGCAGAGACTTGGCTATAGGAGCAGGG17630.14922998007445418No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTGGTTGGAAAGCC17400.14728313404965981No Hit
AACAGAGAATCTCCATTTTAGCACCATGTCGTCAGTGTTTTGTCATATTT17060.14440518775213773No Hit
ACTAGATATTCCTTGTCATTATCTGCACCCGTACCCAAAAGTGAAACATT16780.14203511433064894No Hit
AGGGATGCAAAGGCCTCATCTCGCTGGCAGGGATGTAGAACCATGTCGTC16720.14152724145461562No Hit
AACAGAGAATCTCCATTTTAGCACGACTTTGTCATATTTTTCCCCTCTGT16570.14025755926453234No Hit
AACAGAGAATCTCCATTTTAGCACAGACTTTGTCATATTTTTCCCCTCTG16380.13864929515709354No Hit
ACAAGTCAACAACCCCCACACACTGACTTCCATTTTCTACTTTTTCTGAG16360.13848000419841577No Hit
AGGGATGCAAAGGCCTCATATCTCGCTGGCAGGGATGTAGAACCATGTCG16330.1382260677603991No Hit
AAGGGTGCAGTTATGCCTCACTCAAAATGCCGTTTTCTTGTAGAACCATG16200.13712567652899363No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC16150.1367024491322992No Hit
ATGAGGTACTCGTCGGCATCGCGTCTAGAAGTTGGCTATAGGAGCAGGGG15920.13475560310750484No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT15740.13323198447940493No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC15720.13306269352072714No Hit
CCAACTGGCCAAGACCTGCTACCGTAGCGATGGTGAGCAGCTTGTAGAAC14830.12552924585956637No Hit
GGACCTAATTACGAGTGACACTGTAGAACCATGTCGTCAGTGTAGATCTC14290.1209583899752666No Hit
CCATGGGACTGACTTTCTGCTACCGCCAAGCAATGGATGATTTGATGTAG14250.12061980805791106No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT14230.12045051709923327No Hit
CCAACTGGCCAAGACCTGCGGTAGCGATGGTGAGCAGCTTGTAGAACCAT14130.11960406230584443No Hit
AACAGAGAATCTCCATTTTAGCACCAGAGACTTTGTCATATTTTTCCCCT14020.1186729620331167No Hit
TCCCTGCTTCTGTCTCCTACCGTAGAAGCATGTCGTCAGTGTGCCATTTT13870.11740327984303342No Hit
GGCCTCAGTACAAACTCATTAGCCAGAGACTTGGTCTGGACCTAATTACG13510.11435604258683357No Hit
CCACACAGCAAAGCAGAGACGTTAACTGGCAATTGTGATGTAGAACCATG13470.11401746066947802No Hit
AACAGAGAATCTCCATTTTAGCACCAAGTTTGTCATATTTTTCCCCTCTG13280.11240919656203921No Hit
TCATTACACCAGTTCGTCCCTGCTACCGGTGGTAAAAAGATAACCTCAGA13220.1119013236860059No Hit
ACTAGATATTCCTTGTCATTATCTGCACCGTAATTACCCAAAAGTGAAAC13140.11122415985129482No Hit
AAGGGTGCAGTTATGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTG13030.11029305957856708No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA12880.1090233773884838No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCCGCCCCCAGGGAGCACT12820.1085155045124505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)