FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910757
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC586773.070877144503461No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG489852.563643623966836No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC483502.5304107220332046No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA451942.365240582659124No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA417692.1859922533320564No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA406202.125859018179706No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG400542.0962372504719333No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT399112.0887533056270366No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG389442.0381450911863728No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT354461.8550762865189034No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG335891.7578896740925192No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA317871.6635815019910956No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA309541.6199862148876074No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG304381.592981211111617No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT273001.428753106752978No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT263221.3775692042473218No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT256071.340149480022839No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC248161.2987522746220477No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG245991.2873955191581137No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT239171.2517028591286072No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC238401.2476730426736629No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA229641.201827338588842No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT208521.09129523011037No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT206221.0792581160241728No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC204701.0713031536715554No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC203701.0660696258079911No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC202141.0579053223408315No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT197181.031947024137554No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT192091.005308367312013No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT190170.9952599938139701No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA180840.9464311788469176No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT169710.8881820137254501No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG162010.8478838491760072No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT159340.8339103297802913No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC151260.7916234246426939No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA138490.7247912738249814No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT135700.7101897310856378No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA133460.6984666286712544No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC122240.639746446042066No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT121400.6353502826366723No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT112970.5912316427468276No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA102900.5385300171607379No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT101110.5291620022849582No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT95290.49870287011901565No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT92110.48206025151288207No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC88100.4610738047799903No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA86340.4518627957401177No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA82400.43124269595767545No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT78910.412977683713837No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG77320.4046563744107702No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC69060.36142743425773133Illumina Single End PCR Primer 1 (100% over 21bp)
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG63440.33201500766450154No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA62160.32531609199913963No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT57660.3017652166131015No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC54820.2869019974805797No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT48740.2550821480701104No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA44660.2337293543867692No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT43360.22692576816413598No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG40060.2096551262143747No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA39950.2090794381493827No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA36830.19275083121506292No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTTTTT36600.1915471198064432No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA34950.18291179883156256No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG33550.17558485982257294No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA33120.1733344428412404No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT30670.16051229957550855No Hit
GTATCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT30130.15768619452918398No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT29230.15297601945197636No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA29050.15203398443653485No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC27470.14376501041210368No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA25080.13125687881818565No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG24800.12979149101638776No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC24530.12837843849322544No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC24300.12717472708460573No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC22890.11979545279698046No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG22770.11916742945335278No Hit
CCATGGGACTGACTTTCTGCTACCGCCAAGCAATGGATGATTTGATGTAG22450.11749270053701229No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG22400.11723102414383409No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT21620.11314887241025415No Hit
CCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTCCTGAAAACAACG19560.10236780501131228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)