FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181480
Sequences flagged as poor quality0
Sequence length150
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT1856610.230328410844171No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT148828.20035265594005No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG132207.284549261626625No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA120456.63709499669385No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC115966.389684813753583No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT95445.25898170597311No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC93165.133348027330835No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG70383.8781132907207403No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTA58553.2262508265373593No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT53212.9320035265594004No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG36211.9952611858055982No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA31171.7175446330174124No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC27181.4976856953934317No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA27061.4910733965175225No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA25651.4133788847255895No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT22791.2557857615164205No Hit
GGGGTCGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGT22261.2265814414811549No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTAGTA16620.915803394313423No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTAGCCAGTAAT11630.6408419660568657No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC11400.6281683932113732No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT10770.5934538241128499No Hit
TCTGTATCAGGCAAAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTAC10620.5851884505179634No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG8810.48545294247299975No Hit
GGGGTCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAG8150.44908529865549923No Hit
TCTGTATCAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGT8120.447432223936522No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT7390.407207405774741No Hit
GGGGTCAGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGT7120.3923297333039454No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG5990.33006391888913383No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG5700.3140841966056866No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAGTA5440.29975754904121665No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA4130.2275732863125413No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA3500.19285871721401807No Hit
TCTGTATCAGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTA3420.18845051796341195No Hit
GGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGTAGCCAG3320.18294026890015427No Hit
GGAAACCGTAGCTGACCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTA2900.15979722283447212No Hit
GGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGTAGCCA2840.15649107339651752No Hit
AGCTGCTCACCATCGCTACCGTAAAGTCTTGGCCAGTTGGAGACCAAGTC2690.14822569980163103No Hit
TCTGTATCAGGCAGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTAC2500.1377562265814415No Hit
GGAAACCGTAGCTGCCCTGGGTCTGAGACCAAGTCTCTGCTACCGTAGTA2500.1377562265814415No Hit
GGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGTAGCC2390.13169495261185804No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGGAGACCAAGTCTCTGCT2320.1278377782675777No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT2290.1261847035486004No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACCTTACTTTCATTG2260.12453162882962311No Hit
TCTGTATCAGGCAAAGTCATTGTCTTTGAGGCAGACCAAGTCTCTGCTAC2030.1118580559841305No Hit
TCTTAAATGGCTACGACCCAGTCTCGGCTACCTGTTAAAGAATCATCTGG1820.10028653295128939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)