FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181480
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG131437.242120343839542No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG120006.6122988759091905No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA114166.290500330614944No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC108805.9951509808243335No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA102185.630372492836677No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC90674.99614282565572No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA82094.523363456028213No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT67793.735397839982367No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA57183.1507604143707297No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA53332.93861582543531No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG52812.90996253030637No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG32291.779259422525898No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT26581.464624201013886No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA24401.344500771434869No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA23411.289949305708618No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT22901.261847035486004No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT22061.2155609433546397No Hit
CTTGGGCCTGTGTTATCTCCGACCCCTGTAGAACCATGTCGTCAGTGTAG21941.2089486444787305No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA16050.8843949746528542No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC12120.6678421864668282No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG11610.6397399162442142No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG11520.6347806920872824No Hit
GCCTCAAAGACAATGGCTTTGCCTGATACAGATGTAGAACCATGTCGTCA10690.5890456248622438No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT9510.5240246859158034No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC9370.5163103372272427No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC8170.4501873484681508No Hit
GCCTCAAAGACAATGGCTGATACAGATGTAGAACCATGTCGTCAGTGTAG7950.4380648005289839No Hit
CTTGGGCCTGTGTTATCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTA7920.43641172581000665No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT7800.42979942693409745No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC7420.4088604804937183No Hit
CTTGGGCCTGTGTTATCTCTGACCCCTGTAGAACCATGTCGTCAGTGTAG6920.38130923517743004No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA5300.29204320035265596No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG5180.2854309014767467No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA5140.28322680185144367No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA4020.22151201234295792No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGAGACAGAGATTT4010.22096098743663214No Hit
GCCTCAAAGACAATGGCTCTGATACAGATGTAGAACCATGTCGTCAGTGT3370.18569539343178312No Hit
CTTGGGCCTGTGTTATCTCCTGTAGAACCATGTCGTCAGTGTAGATCTCG3190.17577694511791933No Hit
AAGACCCAGGTCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA2840.15649107339651752No Hit
CTTGGGCCTGTGTTATCTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC2800.15428697377121445No Hit
CCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG2660.14657262508265373No Hit
CCAACTGGCCAAGACTTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA2600.14326647564469913No Hit
GCCTCAAAGACAATGGCTCTGCCTGATACAGATGTAGAACCATGTCGTCA2490.1372052016751157No Hit
CAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA2400.13224597751818382No Hit
CTTGGGCCTGTGTTATCTCCCCTGTAGAACCATGTCGTCAGTGTAGATCT2370.13059290279920652No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT2270.12508265373594887No Hit
CCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT2200.1212254793916685No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA2100.11571523032841084No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT2080.1146131805157593No Hit
GCCTCAAAGACAATGACTTTGCCTGATACAGATGTAGAACCATGTCGTCA2000.11020498126515318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)