FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642916
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG306684.770141044864337No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC210913.2805218722196985No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA209273.2550130965787134No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG197883.0778515389257697No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT193423.008480112487479No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA180292.804254366044709No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC162172.5224135034747928No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG153922.394091918695444No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG151322.3536511768255886No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT143432.2309290793820655No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA138072.147558934604209No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA137782.143048236472572No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT134732.0956081354329337No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC105041.633805971542161No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACCATATGATCAT94321.4670656819864494No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA92911.4451343565877968No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA90681.4104486433686516No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCCAGGTG90451.4068711931263183No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT83751.3026585121539984No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC76881.1958016288286495No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA74521.1590938785160114No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG74251.1548942630141417No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA74031.1514723540866925No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG68981.0729239900702423No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC68151.0600140609348656No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC65821.0237729345668796No Hit
ATACGGCCAGGCATTGAAGTACACTCACGACAGGGGCCAGGAGGAGACCA65451.0180179059161694No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT60920.9475576902736905No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT58250.9060281591996465No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG56560.8797416769842406No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT54710.8509665337306895No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA53590.8335459064636749No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA53060.8253022167748197No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC50270.7819061899221671No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG47730.742398695941616No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG47000.7310441799550798No Hit
TTTCGCTTCCCACAGGTCTCTACGGAAGGCAGTCTGGCTGAGACCAAGTC46210.7187564160792389No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG38440.5979008144143247No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT38160.593545657597571No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG36560.5686590472161216No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC33470.5205967809169472No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC33450.5202856982871791No Hit
GGGGTCAGAGGCAAGCAGGACATGGGAGATAACACAGGCCCAAGAGACCA32600.507064686522034No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT29780.46320203572472923No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT28830.4484256108107435No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC28480.44298166478980144No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA27940.4345824337860622No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACCA27800.43240485537768536No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA27240.42369454174417803No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT27020.4202726328167288No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT26270.4086070342004243No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC24190.37625444070453995No Hit
AGCTGCTCACCATCGCTATAGCAGGTCTTGGCCAGTTGGAGACCAAGTCT23700.368632916275221No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG23260.3617890984203224No Hit
GGAATCCTATGGCTTTCCAACCTACAGCAACAGAGGAGGGGGAGACCAAG20200.31419345606580024No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT19160.29801715931785805No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATTTGGACCTGGGTCTTCAGTGAAC17830.27733016443827807No Hit
TGTGCTGTGACTGCTTGTAGAAGTGTACAGAGTTGAAAGTCAGGGCAAGA16050.24964381038891553No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC15710.2443554056828575No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTAGAGGACCGTCGCTTGGTG15440.24015579018098787No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT13820.2149580971697702No Hit
TTTCGCTTCCCACAGGTCTCTGCTACGGAAGGCAGTCTGGCTGAGACCAA12890.2004927548855527No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACCATATGATCATCT12590.1958265154390309No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTACCA12170.18929378021390042No Hit
ACTTCTACCGTGCCCTGATGGATGAGGAAGACATGGACGACGTGGTGGAT11650.18120563183992933No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG11060.1720286942617698No Hit
ATACGGCCAGGCATTGAAGTCTCGTGGAAGCCAGCCCCTCAGGGCAACTG10600.16487379377710307No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG10540.16394054588779872No Hit
GTGTCACTCGTAATTAGGTCCAGACCAAGTAGCTAATGAGTTTGTACTGA10420.16207405010919002No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA9480.1474531665100884No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG9440.14683100125055218No Hit
GGAAACCGTAGCTGCCCTGTTACCGTATTCATCTGGACCTGGGTCTTAGA9080.14123151391472602No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCAGGCTCTCTTTAACAATT8980.13967610076588544No Hit
TCCCTGCCAGCGAGATGGGATGAGGCCTTTGCATCCCTAGACCAAGTCTC8870.13796514630216078No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCCCCCAG8820.1371874397277405No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT8550.13298782422587088No Hit
GCGTGGACAACCCCCACCACTGACTCTGATCCAGAAGACCAAGTCTCTGC8430.13112132844726215No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACTTCATCTGGACCTGGGTCTTA7750.12054451903514612No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACCATATGATCAT7190.11183420540163877No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACCATATGATCATCTCG7090.11027879225279817No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAAGGT6630.10312389176813144No Hit
GGGGTCAGAGGCAAGCAGGAGGAGATAACACAGGCCCAAGAGACCAAGTC6490.10094631335975461No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA6450.10032414810021838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)