FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642916
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC306674.769985503549453No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC194823.0302558965712474No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA175642.7319276546236213No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA169032.6291148454852578No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG157032.4424652676243865No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG154402.4015579018098787No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT153412.386159311636357No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA136942.129982766022311No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC132852.0663663682347306No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA131022.0379023076109477No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA123321.9181354951502219No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA103651.6121857287732768No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG95221.4810644003260145No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG94641.4720430040627392No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT91821.4281803532654342No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT90861.4132483870365646No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC88731.38011808696626No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA88661.3790292977620715No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT81351.265328596581824No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG70421.0953219394135472No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT68991.0730795313851265No Hit
CCTCCTGGCCCCTGTCGTGAGTGTACTTCAATGCCTGGCCGTATTGTAGA64110.9971753697217055No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT63410.9862874776798213No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT57410.8929626887493856No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG54590.8491000379520809No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC53080.8256132994045878No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA53010.8245245102003994No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT52860.8221913904771385No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG52670.8192361054943414No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG52430.815503113937124No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC50920.7920163753896309No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT50350.7831505204412396No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA48970.7616858189872394No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT46630.7252891513043694No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC46290.7200007465983115No Hit
CAGCCAGACTGCCTTCCGTAGAGACCTGTGGGAAGCGAAATGTAGAACCA44900.6983805038294272No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA43640.6787822981540357No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG41890.6515625680493252No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG37200.5786136913687014No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT35500.5521716678384112No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT35460.551549502578875No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA34400.5350621232011646No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA32320.5027095297052803No Hit
CTTGGGCCTGTGTTATCTCCCATGTCCTGCTTGCCTCTGACCCCTGTAGA31780.4943102987015411No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT31610.4916660963485121No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA29150.4534029328870335No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC28830.4484256108107435No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT27310.42478333094836646No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA27020.4202726328167288No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA26410.41078461260880117No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC26290.4089181168301924No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT25570.39771914215854015No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT24290.37780985385338056No Hit
CCAACTGGCCAAGACCTGCTATAGCGATGGTGAGCAGCTTGTAGAACCAT23470.36505546603288763No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG21520.33472490963049606No Hit
CCCCCTCCTCTGTTGCTGTAGGTTGGAAAGCCATAGGATTCCTGTAGAAC19790.30781626215555374No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATGGCGCCTCTG19630.3053276011174088No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT19460.3026833987643798No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT18910.29412862644575655No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT16870.26239819820940835No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA15680.2438887817382053No Hit
TGCCCTGACTTTCAACTCTGTACACTTCTACAAGCAGTCACAGCACATGT15570.24217782727448067No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC13740.21371376665069775No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT13580.2112251056125528No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC12810.19924842436648024No Hit
CAGCCAGACTGCCTTCCGTAGCAGAGACCTGTGGGAAGCGAAATGTAGAA12620.1962931393836831No Hit
GTTCAAATGAGTAGACACAGCTTTAGAGAGAGAGACAGAGATTTCATACC12360.19224906519669754No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA11970.18618295391621922No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA11430.17778372291248No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA10920.16985111585339296No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG10830.16845124401943645No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTTTTT10150.1578744346073204No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA10140.15771889329243635No Hit
GGCCTCAGTACAAACTCATTAGCTACTTGGTCTGGACCTAATTACGAGTG9970.15507469093940732No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAGGATTTACCTCTA9340.1452755881017116No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGCGACAGAAAGGTAAAGAAGAGCA9130.14200922048914633No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT9110.1416981378593782No Hit
AAGACCCAGGTCCAGATGAATACGGTAACAGGGCAGCTACGGTTTCCTGT8950.13920947682123325No Hit
AGGGATGCAAAGGCCTCATCCCATCTCGCTGGCAGGGATGTAGAACCATG8890.1382762289319289No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC8630.13423215474494335No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAACCTACCAGGGCA8550.13298782422587088No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG8550.13298782422587088No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT8470.1317434937067984No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT8350.12987699792818969No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAAACCAAAATCAC8290.12894375003888534No Hit
TCTGGATCAGAGTCAGTGGTGGGGGTTGTCCACGCTGTAGAACCATGTCG8260.12847712609423315No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT8050.1252107584816679No Hit
CCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT7790.12116668429468236No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA7760.12070006035003018No Hit
AAGACCCAGGTCCAGATGAAGTCTAAGACCCAGGGCAGCTACGGTTTCCT7560.11758923405234899No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGGGCA7540.11727815142258087No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC7360.11447840775466779Illumina Single End PCR Primer 1 (100% over 21bp)
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG7190.11183420540163877No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG7030.10934554436349384No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC6870.10685688332534887No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTAACTGTACCACCATCCAC6750.10499038754674017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)