FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575550
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA312305.426114151681001No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA256044.448614368864565No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG245304.2620102510642No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT217593.780557727391191No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA215833.7499782816436458No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG200053.4758057510207627No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC197973.4396664060463906No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG165692.8788115715402656No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC163042.8327686560681085No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA124162.1572409000086874No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT123922.1530709755885677No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA113161.966119364086526No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC107921.8750760142472418No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA106391.8484927460689775No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT100631.7484145599861005No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC92711.6108070541221442No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG89681.5581617583181304No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG88731.5416558074884892No Hit
GGAATCCTATGGCTTTCCAACCTACAGCAACAGAGGAGGGGGAGACCAAG83331.4478325080357919No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG72381.257579706367822No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTATAACACC70601.2266527669185996No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT70271.220919120840935No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGATCCTATAAT69231.2028494483537484No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC69121.200938232994527No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC65861.144296759621232No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT65611.1399530883502735No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC59981.0421336113282946No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG59321.030666319172965No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG58391.0145078620450003No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGAAGGCTG55690.9675962123186518No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT46360.8054904004864912No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA42730.7424202936321779No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT41060.7134045695421771No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACGA38850.6750065155069065No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA37110.6447745634610372No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG34610.6013378507514551No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC31630.5495612892016332No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG28450.49430979063504477No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC27870.48423247328642166No Hit
GGAATCCTATGGCTTTCCAACCGTACAGCAACAGAGGAGGGGGAGACCAA22280.38710798366779603No Hit
GAGAAAGCCCCCCTACTGCTACCCTTCCACTCGGATAAGATGCTAGACCA18500.32143167405090783No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC17510.30423073581791327No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT15120.2627052384675528No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC14640.25436538962731303No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA14300.24845799669880983No Hit
TGACCAATGGCTAAGTGAAGATGTATTCCTCCAATTCAGGACCCACACGA12280.21336113282946745No Hit
GCGTGGACAACCCCCACCACTGACTCTGATCCAGAAGACCAAGTCTCTGC11470.19928763791156284No Hit
GGAAACCGTAGCTGCCCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTC11290.1961601945964729No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC10840.18834158630874814No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACGATCCTATAATCT10300.1789592563634784No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGGAGATAAC9890.17183563547910694No Hit
GCAGCTTCTGCCATCTCTCTTCTCCTTTTTCTTCAGCCACAGGCTCCCAG9390.1631482929371905No Hit
GGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACGATCCT9120.15845712796455563No Hit
CAGCCTCTGGCATTCTGAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCT8820.1532447224394058No Hit
GGGGTCGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACG8550.1485535574667709No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTCCATACCAGGACCA8120.14108244288072277No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA7450.12944140387455477No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTACACCA7220.12544522630527322No Hit
GGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACGATCC7170.12457649205108157No Hit
GGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACGATCCTA6920.12023282078012336No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA6800.11814785857006342No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACGA6390.11102423768569193No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC6190.10754930066892539No Hit
GGAAACCGTAGCTGCCCTGTTAAGGAATCATCTGGACCTGGGTCTTAGAC6130.1065068195638954No Hit
TCTGTATCAGGCAAAGTCATAGAATCATTTTCATGCTCTCTTTAACAATT6030.1047693510555121No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGATA5880.10216314829293718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)